Invest. Ophthalmol. Vis. Sci ;53(4): doi: /iovs Figure Legend:

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Lactate-H+ Transport Is a Significant Component of the In Vivo Corneal Endothelial Pump Invest. Ophthalmol. Vis. Sci.. 2012;53(4):2020-2029. doi:10.1167/iovs.12-9475 Figure Legend: Lactate-induced acidification in BF and BR Ringer's solution and in the presence of acetazolamide (ACTZ), a CA inhibitor. These are representative traces of the change in pHi induced by 20 mM lactate in BF and BR medium and in the absence and presence of 100 μM ACTZ. Arrows indicate where lactate pulses were given. (A) There was significant decrease in pHi in when lactate was perfused for 1 minute on either the BL or apical surface in BF Ringer's solution. The results are from two different CE samples. There was little (BL surface) or no (Apical) LIA when the CE were perfused in BR Ringer's solution. (B) The rate of acidification was not affected by ACTZ in BF Ringer's solution; however, in BR Ringer's solution, acidification was slightly faster (from 0.0006 to 0.0008 pH units/sec). The dashed lines are estimates of the slope. Date of download: 10/17/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.