A case of testicular infarction from the complications of Klebsiella oxytoca induced acute epididymitis Wonae Lee, Heeyoon Park, Gilho Lee Journal of.

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A case of testicular infarction from the complications of Klebsiella oxytoca induced acute epididymitis Wonae Lee, Heeyoon Park, Gilho Lee Journal of Infection and Chemotherapy Volume 22, Issue 4, Pages 254-256 (April 2016) DOI: 10.1016/j.jiac.2015.09.011 Copyright © 2015 Terms and Conditions

Fig. 1 Color Doppler sonography findings at two epididymitis episodes. A. Color Doppler sonography of left scrotum at first admission revealed left epididymal swollen and increased vascularity. However, the testicle revealed well preserved vascularity. B. Thirteen days later, the Doppler sonography revealed a small sized, irregular-shaped and heterogeneous left testis with absent blood flow. Journal of Infection and Chemotherapy 2016 22, 254-256DOI: (10.1016/j.jiac.2015.09.011) Copyright © 2015 Terms and Conditions

Fig. 2 Macroscopically, the sectioned surface of the testis is yellowish and white in color, friable and totally necrotic. The capsule of the testis is markedly thicker with fibrous adherence (A). Microscopically, seminiferous tubules and interstitium are entirely effaced by intense acute inflammation (Hematoxylin-eosin stain, original magnification ×200) (B). Journal of Infection and Chemotherapy 2016 22, 254-256DOI: (10.1016/j.jiac.2015.09.011) Copyright © 2015 Terms and Conditions

Fig. 3 Analysis of the 16S rRNA gene sequences. The electropherograms reveal variations in the 16S rRNA genes sequence from Klebsiella oxytoca strain MTJW-7 (GenBank accession no KM516015.1), Klebsiella sp. Y1 (HQ616650.1), E. coli (J01859.1), and P. aeruginosa (M34133.1), and our case. The box represents the dissimilar DNA sequences among five different isolates. The arrow indicates the 451th nucleotide site of gene accession no. J01859.1. Journal of Infection and Chemotherapy 2016 22, 254-256DOI: (10.1016/j.jiac.2015.09.011) Copyright © 2015 Terms and Conditions

Fig. 4 The results of polymerase chain reaction by using Klebsiella oxytoca specific and Klebsiella pneumoniae specific primers. We used DNA templates from the urine sample and epididymitis tissue sample. Lanes 1–4 show the results of unique gene of Klebsiella oxytoca (peh X) and lanes 5–8 show the results of amplification of unique gene of Klebsiella pneumoniae (rpo B). Lanes 1 and 5 reveal the results from the urine specimens; lanes 2 and 6 reveal the results from the epididymitis tissues; lanes 3 and 7 present the results of 10 times dilution from the stored DNA sample from the epididymitis tissue in lanes 2 and 6; lanes 4 and 8 present the results of 40 times dilution from the stored DNA sample from the epididymitis tissue in lanes 2 and 6. M represents 100 bp DNA marker. Journal of Infection and Chemotherapy 2016 22, 254-256DOI: (10.1016/j.jiac.2015.09.011) Copyright © 2015 Terms and Conditions