PI 3-kinase inhibition has no effect on ANG II induced extracellular recognition kinase (ERK) 1/2 activation. PI 3-kinase inhibition has no effect on ANG II induced extracellular recognition kinase (ERK) 1/2 activation. VSMC were pretreated with 1–20 μM LY-294002 for 45 min before treatment with ANG II for 5 min. Immunoblot analysis was performed with anti-phospho-specific ERK1/2 antibodies (1:5,000). Petra Rocic et al. Am J Physiol Cell Physiol 2001;280:C90-C99 ©2001 by American Physiological Society