Time course of vascular reactivity to contracting and relaxing agents after endothelial denudation by balloon angioplasty in rat carotid artery Laura.

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Time course of vascular reactivity to contracting and relaxing agents after endothelial denudation by balloon angioplasty in rat carotid artery Laura Lippolis, Raffaella Sorrentino, Ada Popolo, Pasquale Maffia, Caterina Nasti, Roberta d’Emmanuele di Villa Bianca, Stefania Marzocco, Giuseppina Autore, Aldo Pinto Atherosclerosis Volume 171, Issue 2, Pages 171-179 (December 2003) DOI: 10.1016/j.atherosclerosis.2003.07.012

Fig. 1 Contractile responses to phenylephrine (PE 0.3μM) of carotid artery rings at different days from angioplastic balloon injury compared to uninjured carotid (UC). Results are expressed as mean±S.E. mean of dine/mg of fresh tissue collected by 8–14 animals. Data were analysed by analysis of variance (one-way ANOVA) and Bonferroni’s multiple comparison test and P<0.05, P<0.01 and P<0.001 compared to UC. Atherosclerosis 2003 171, 171-179DOI: (10.1016/j.atherosclerosis.2003.07.012)

Fig. 2 Contractile responses to angiotensin II (ATII 0.1μM) of mechanically injured carotid artery rings collected at different time from angioplastic balloon injury compared to uninjured carotid (UC). Results are expressed as mean±S.E. mean of dine/mg of fresh tissue (n=4–12). Data are analysed by analysis of variance (one-way ANOVA) and Bonferroni’s multiple comparison test and P<0.05, P<0.01 and P<0.001 compared to UC. Atherosclerosis 2003 171, 171-179DOI: (10.1016/j.atherosclerosis.2003.07.012)

Fig. 3 Contractile responses to PGH2/TXA2 receptor agonist (U46619 0.1μM) of mechanically injured carotid artery rings collected at different time from angioplastic balloon injury compared to uninjured carotid (UC). Results are expressed as mean±S.E. mean of dine/mg of fresh tissue (n=4–6). Data are analysed by analysis of variance (one-way ANOVA) and Bonferroni’s multiple comparison test and P<0.05, and P<0.01 compared to UC. Atherosclerosis 2003 171, 171-179DOI: (10.1016/j.atherosclerosis.2003.07.012)

Fig. 4 Contractile responses to KCl (60mM) of carotid artery rings at different time from angioplastic balloon injury compared to uninjured carotid (UC). Results are expressed as mean±S.E. mean of dine/mg of fresh tissue. Data are analysed by analysis of variance (one-way ANOVA) and Bonferroni’s multiple comparison test and all points resulted statistically significant ( P<0.001; n=5–8) compared to UC rings. The KCl-induced contraction at 28 days resulted significantly higher compared to 1 (#P<0.05), 7 (§§P<0.01) and 14 (&P<0.05) days. Atherosclerosis 2003 171, 171-179DOI: (10.1016/j.atherosclerosis.2003.07.012)

Fig. 5 Contractile responses to calcium ionophore (A23187; 1μM) of mechanically injured carotid artery rings collected at different time from angioplastic balloon injury compared to uninjured carotid (UC). Results are expressed as mean±S.E. mean of dine/mg of fresh tissue (n=4–6). Data are analysed by analysis of variance (one-way ANOVA) and Bonferroni’s multiple comparison test and P<0.05, P<0.01 and P<0.001 compared to UC. Atherosclerosis 2003 171, 171-179DOI: (10.1016/j.atherosclerosis.2003.07.012)

Fig. 6 (A) Vasodilator responses curve to EDRF release induced by cumulative administration of Acetylcholine (ACh; 10nM–10μM), in presence of indomethacin (10μM), of mechanically injured carotid artery rings collected at different time from angioplastic balloon injury compared to uninjured carotid (UC). Results are expressed as mean±S.E. mean of percentage of relaxation. Curves were compared by analysis of variance (two-way ANOVA) and following results are obtained: 14 and 21 days P<0.0001 (n=5) and P<0.001 (n=4), respectively, compared to rings obtained from UC (n=13). At 28 days (n=4) no statistical differences were found compared to UC tissue. (B) Increase in PE (0.3μM) induced contraction by l-NAME (100μM). Data are analysed by analysis of variance (one-way ANOVA) and Bonferroni’s multiple comparison test and P<0.05 and P<0.01 compared to UC. Atherosclerosis 2003 171, 171-179DOI: (10.1016/j.atherosclerosis.2003.07.012)

Fig. 7 Vasodilator responses curve to EDHF release induced by cumulative administration of acetylcholine (ACh; 10nM–10μM), in presence of indomethacin (10μM) and l-NAME (100μM), of mechanically injured carotid artery rings collected at different time from angioplastic balloon injury compared to uninjured carotid (UC). Results are expressed as mean±S.E. mean of percentage of relaxation. Curves were compared by analysis of variance (two-way ANOVA) and all curves of injured carotids were statistically (P<0.01; n=3–7) significant compared to UC. Atherosclerosis 2003 171, 171-179DOI: (10.1016/j.atherosclerosis.2003.07.012)

Fig. 8 Vasodilator responses curve to sodium nitroprusside (SNP; 1nM–3μM) of mechanically injured carotid artery rings collected at different time from angioplastic balloon injury compared to uninjured carotid (UC). Results were expressed as mean±S.E. mean of percentage of relaxation. Curves were compared by analysis of variance (two-way ANOVA) and following results are obtained: 14 (n=3) and 21 (n=3) days P<0.0001 vs. UC rings (n=16), 28 days (n=3) P<0.05 vs. UC and 28 days vs. 14 and 21 days P<0.0001. Atherosclerosis 2003 171, 171-179DOI: (10.1016/j.atherosclerosis.2003.07.012)

Fig. 9 Vasodilator responses curve to cumulative administration of diazoxide (DZX; 3μM–300μM) on mechanically injured carotid artery rings collected at different time from angioplastic balloon injury compared to uninjured carotid (UC). Results are expressed as mean±S.E. mean of percentage of relaxation (n=5). Curves were compared by analysis of variance (two-way ANOVA) and the response at 7 days was statistically significant (P<0.0001) compared UC whereas at 14, 21 and 28 days it was not different from that obtained on UC rings. Atherosclerosis 2003 171, 171-179DOI: (10.1016/j.atherosclerosis.2003.07.012)

Fig. 10 (A) Representative blot of MHC expression in SMC at 14 and 28 days after angioplastic balloon. (B) Densitometry of Western blotting to detect expression of MHC in UC and IC at 14 and 28 days after angioplastic balloon. §P<0.0005 and #P<0.005 vs. to UC, and P<0.05 vs. 28 days. Atherosclerosis 2003 171, 171-179DOI: (10.1016/j.atherosclerosis.2003.07.012)