Volume 14, Issue 6, Pages 454-458 (November 2014) Electron-microscopic evidence of mitochondriae containing macroautophagy in experimental acute pancreatitis: Implications for cell death  Tony George Jacob, Vipin Iyani Sreekumar, Tara Sankar Roy, Pramod Kumar Garg  Pancreatology  Volume 14, Issue 6, Pages 454-458 (November 2014) DOI: 10.1016/j.pan.2014.08.009 Copyright © 2014 IAP and EPC Terms and Conditions

Fig. 1 Photomicrographs of H&E stained, 5μ thick sections of the pancreas derived from mice of Groups 2 (‘A’ and ‘B’) and 3 (‘C’ and ‘D’). ‘C’ when compared to ‘A’ shows more inflammatory infiltration (arrows) and even sites of microhemorrhages (He). G2 also shows more apoptosis (Ap) than necrosis (Ne). Pancreatology 2014 14, 454-458DOI: (10.1016/j.pan.2014.08.009) Copyright © 2014 IAP and EPC Terms and Conditions

Fig. 2 Electron micrograph of a normal pancreatic acinar cell showing normal mitochondria [M], nucleus [N], zymogen granules [Z] and rough endoplasmic reticulum [rER]. Scale bar = 1 μm. Pancreatology 2014 14, 454-458DOI: (10.1016/j.pan.2014.08.009) Copyright © 2014 IAP and EPC Terms and Conditions

Fig. 3 Electron micrograph of the pancreatic acinar cell from a mouse that received 4 injections of caerulein showing zymogen granules [Z], mitochondria [M] and a large autophagosome [arrows] containing abnormal mitochondria [aM], organellar membranes [OM] and zymogen granules [Zy]. N.B. the zymogen granule marked with ‘Z’ appears to be docking on the autophagosomal membrane. Scale bar = 1 μm. Pancreatology 2014 14, 454-458DOI: (10.1016/j.pan.2014.08.009) Copyright © 2014 IAP and EPC Terms and Conditions

Fig. 4 Photomicrograph of the TEM of the pancreas derived from an animal that received 8 injections of caerulein showing a large autophagosome [Au] containing a mitochondria [M] showing dilated and abnormal cristae [inset]. The dashed arrow indicates a nuclear fragment within the autophagosome. Scale bar = 1 μm [inset = 200 μm]. Pancreatology 2014 14, 454-458DOI: (10.1016/j.pan.2014.08.009) Copyright © 2014 IAP and EPC Terms and Conditions

Fig. 5 Electron micrograph of a pancreatic acinar cell from a mouse that received 8 hourly injections of caerulein, showing a nucleus [N] with peripheral condensation of chromatin indicative of apoptosis, deformed rough endoplasmic reticulum [rER] and an autophagosome [inset] containing zymogen granules [Z] and mitochondria edema and deformed cristae. Scale bar = 2 μm. Pancreatology 2014 14, 454-458DOI: (10.1016/j.pan.2014.08.009) Copyright © 2014 IAP and EPC Terms and Conditions

Fig. 6 Electron micrograph of a pancreatic acinar cell from a mouse that received 8 hourly injections of caerulein, showing an autophagosome containing abnormal mitochondriae (aM). The double membrane of the autophagosome can be appreciated (arrow). Pancreatology 2014 14, 454-458DOI: (10.1016/j.pan.2014.08.009) Copyright © 2014 IAP and EPC Terms and Conditions

Fig. 7 Photomicrographs of H&E stained, 5μ thick sections of the pancreas derived from rats that were given saline infusion into the main pancreatic duct (‘A’ and ‘B’) and Na-taurocholate (‘C’ and ‘D’). The saline infusion produces intralobular edema and mild inflammation (In); whereas infusion with Na-taurocholate produces more edema, inflammation and even microhemorrhages (He). PD = interlobular pancreatic duct. Pancreatology 2014 14, 454-458DOI: (10.1016/j.pan.2014.08.009) Copyright © 2014 IAP and EPC Terms and Conditions

Fig. 8 Transmission electronmicrograph of a section of the pancreas derived from a rat that was given saline infusion into the main pancreatic duct showing a large macroautophagosome containing zymogen granules, dilated RER and mitochondriae. Inset: magnified view of the area in the box, showing the cristae of the mitochondria (M). Pancreatology 2014 14, 454-458DOI: (10.1016/j.pan.2014.08.009) Copyright © 2014 IAP and EPC Terms and Conditions

Supplementary Fig. 1 Transmission electronmicrograph of a section of the pancreas derived from a rat that was given saline infusion into the main pancreatic duct showing a pancreatic acinar cell with two nuclei, expanded RER and apical zymogen granules. Pancreatology 2014 14, 454-458DOI: (10.1016/j.pan.2014.08.009) Copyright © 2014 IAP and EPC Terms and Conditions

Supplementary Fig. 2 Transmission electronmicrograph of a section of the pancreas derived from a rat that was given Na-taurocholate infusion into the main pancreatic duct showing a large macroautophagosome (Au). The arrows indicate the double-layered membrane that is enclosing the autophagosome. ‘M’ indicates normal mitochondriae. Pancreatology 2014 14, 454-458DOI: (10.1016/j.pan.2014.08.009) Copyright © 2014 IAP and EPC Terms and Conditions

Supplementary Fig. 3 Electron micrograph of a pancreatic acinar cell from a mouse that received 8 hourly injections of caerulein, showing necrotic morphology-the arrows indicate a disrupted cell membrane and basement membrane; abnormal mitochondriae (M), rough endoplasmic reticulum (rER). The boundaries of the autophagosome have become blurred but they still contain zymogen granules (Z). Pancreatology 2014 14, 454-458DOI: (10.1016/j.pan.2014.08.009) Copyright © 2014 IAP and EPC Terms and Conditions