Skyline not identifying peptides

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Tandem MS (MS/MS) on the Q-ToF2

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Skyline not identifying peptides Eric Mbua

Transition setting:

Peptide sequence: LSELVQAVSDPSSPQYGK found in protein not identified by Skyline

LSELVQAVSDPSSPQYGK: Xcalibur precursor ion (Light) Fragment ion(Light) ion(Heavy) Peptide not identified on Skyline when raw data was imported

Peptide sequence: LASVLGSEPSLDSEVTSK found in protein not identified by Skyline

LASVLGSEPSLDSEVTSK: Xcalibur precursor ion (Light) Fragment ion(Light) ion(Heavy) Peptide not identified on Skyline when raw data was imported

Peptide: YYEEFPINLK No chromatograms for the above peptide when raw data was imported on to Skyline

Peptide: YYEEFPINLK: Xcalibur precursor ion (Light) Fragment ion(Light) precursor ion(Heavy) Fragment ion(Heavy) Heavy labeled peptide observed using Xcalibur; but not when raw data was imported on to skyline