Overview of hybrid capture preparation for sequencing

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Overview of hybrid capture preparation for sequencing Overview of hybrid capture preparation for sequencing. This illustration presents a generalized overview of the process for hybrid capture selection prior to DNA or RNA sequencing. In general, probes are designed for the targeted regions of interest, which can constitute a small number of genes or hotspot loci, up to the full exome (all annotated genes in a genome). Following hybrid capture, the probe:library duplexes are isolated from solution by streptavidin magnetic beads. Release of the library fragments by denaturation is followed by amplification, quantitation, and sequencing. (Used with permission from Illumina, Inc.) Source: Genomics, Williams Hematology, 9e Citation: Kaushansky K, Lichtman MA, Prchal JT, Levi MM, Press OW, Burns LJ, Caligiuri M. Williams Hematology, 9e; 2015 Available at: http://hemonc.mhmedical.com/DownloadImage.aspx?image=/data/books/1581/kau_ch11_f004.png&sec=94302363&BookID=1581&ChapterSecID=94302337&imagename= Accessed: October 18, 2017 Copyright © 2017 McGraw-Hill Education. All rights reserved

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