Volume 105, Issue 5, Pages 1324-1334 (May 1994) Expression of Surface Markers on Alveolar Macrophages From Symptomatic Patients with HIV Infection as Detected by Flow Cytometry  Klaus Wasserman, M.D., F.C.C.P., Marion Subklewe, M.D., Gunter Pothoff, M.D., Norbert Banik, Ph.D., Elisabeth Schell-Frederick, M.D.  CHEST  Volume 105, Issue 5, Pages 1324-1334 (May 1994) DOI: 10.1378/chest.105.5.1324 Copyright © 1994 The American College of Chest Physicians Terms and Conditions

Figure 1 Representative flow cytometry profiles of the expression of five antigens on alveolar macrophages of an HIV-positive patient. Solid lines=FITC-conjugated specific monoclonal antibody; dotted lines=negative isotype control; AM Dot Plot=forward-sideward scatter plot of the total AM population analyzed with outgated lymphocytes (see Methods section). CHEST 1994 105, 1324-1334DOI: (10.1378/chest.105.5.1324) Copyright © 1994 The American College of Chest Physicians Terms and Conditions

Figure 2 Relative linear median fluorescence intensities (RLMFI) (y-axis) for HLA-DR, DP, and DQ in AMs for controls (C) and HIV-infected patients (HIV+). Single values and mean±SEM. Solid points=smokers; circles=nonsmokers. Any value of RLMFI ≤1 indicates absence of specific antigen expression. HLA-DP for smoking HIV-infected patients vs smoking controls: 9.6±1.3 (n=17) vs 6.0±1.1 (n=7), p=0.04; for nonsmoking HIV-infected patients vs nonsmoking controls: 18.8±3.3 (n=7) vs 7.7±1.7 (n=3), p=0.02. HLA-DR for smoking HIV-infected patients vs smoking controls: 23.1±4.1 (n=18) vs 18.0±3.7 (n=7), p=0.39; for nonsmoking HIV-infected patients vs nonsmoking controls: 67.5±11.8 (n=9) vs 48.9±14.2 (n=5), p=0.26. HLA-DQ for smoking HIV-infected patients vs smoking controls: 2.7±0.4 (n=15) vs 2.4±0.4 (n=7), p=0.78; for nonsmoking HIV-infected patients vs nonsmoking controls: 4.3±0.4 (n=9) vs 3.8±1.1 (n=6), p=0.35. CHEST 1994 105, 1324-1334DOI: (10.1378/chest.105.5.1324) Copyright © 1994 The American College of Chest Physicians Terms and Conditions

Figure 3 Relative linear median fluorescence intensities (RLMFI) (y-axis) for CD11c, CD11b, and CD14 in AMs from controls (C) and HIV-infected patients (HIV+). Single values and mean±SEM. Solid points=smokers; circles=nonsmokers. Any value of RLMFI ≤1 indicates absence of specific antigen expression. CD11c for smoking HIV-infected patients vs smoking controls: 8.4±1.1 (n=17) vs 5.1±1.1 (n=7), p=0.04; for nonsmoking HIV-infected patients vs nonsmoking controls: 10.3±2.4 (n=6) vs 4.1±1.4 (n=3), p=0.05. CD11b for smoking HIV-infected patients vs smoking controls: 2.6±0.4 (n=12) vs 1.7±0.3 (n=7), NS; for nonsmoking HIV-infected patients vs nonsmoking controls: 5.5±1.1 (n=5) vs 1.6±0.7 (n=2), p=0.05. CD14 for smoking HIV-infected patients vs smoking controls: 2.0±0.4 (n=17) vs 1.2±0.3 (n=7), p=0.02; for nonsmoking HIV-infected patients vs nonsmoking controls: 2.2±0.5 (n=6) vs 0.5±0.1 (n=3), p=0.02. CHEST 1994 105, 1324-1334DOI: (10.1378/chest.105.5.1324) Copyright © 1994 The American College of Chest Physicians Terms and Conditions

Figure 4 Relative linear median fluorescence intensities (RLMFI) (y-axis) for CD13 and CD33 in AMs from controls (C) and HIV-infected patients (HIV+). Single values and man±SEM. Solid points=smokers; circles=nonsmokers. Any value of RLMFI ≤1 indicates absence of specific antigen expression. CD33 for smoking HIV-infected patients vs smoking controls: 1.6±0.1 (n=15) vs 1.1±0.2 (n=6), p=0.05; for nonsmoking HIV-infected patients vs nonsmoking controls: 1.9±0.3 (n=4) vs 0.8±0.3 (n=2), p=0.05. CHEST 1994 105, 1324-1334DOI: (10.1378/chest.105.5.1324) Copyright © 1994 The American College of Chest Physicians Terms and Conditions

Figure 5 Relative linear median fluorescence intensities (RLMFI) (y-axis) for CD71 in AMs from controls (C) and HIV-infected patients (HIV+). Single values and mean±SEM. Solid points=smokers; circles=nonsmokers. Any value of RLMFI ≤1 indicates absence of specific antigen expression. CHEST 1994 105, 1324-1334DOI: (10.1378/chest.105.5.1324) Copyright © 1994 The American College of Chest Physicians Terms and Conditions

Figure 6 Representative flow cytometry profiles of CD11b antigen expression on large (left) and small (right) AMs taken from an HIV-positive patient. Red area=FITC-conjugated specific monoclonal anti-CD11b-antibody. Black background area=negative isotype control. Fluorescence intensity of large AM was 2.5, and that of small AM was 6.6 RLMFI units (see Methods section). CHEST 1994 105, 1324-1334DOI: (10.1378/chest.105.5.1324) Copyright © 1994 The American College of Chest Physicians Terms and Conditions