A three-dimensional representation of flow cytometry data 3D-CYTOFLOW A three-dimensional representation of flow cytometry data

Flow Cytometry? Flow Cytometer Analyzer High Dimensional Data (.fcs files) Cancer Detection Ryan

Former Solution A set of simple 2D graphs for each pair of properties Smaller samples Less accurate Ryan

Our Solution: SANJAY algorithm Receives a high-dimensional dataset and outputs a 2D/3D representation of it Reduces the amount of information that a pathologist has to understand Minimizes the amount of data lost in translation Ryan

Goal Create a simple, intuitive, and easy to use web portal to allow cancer pathologists to upload flow cytometry data and visualize it in 3D space Ryan

Functionality Two user roles: Admin and Doctor An admin may add/remove doctors A doctor may add/remove patients, upload .fcs files, and view the output of the algorithm Ryan

Registration System The admin adds the doctor, and the doctor receives an email with the token The doctor uses the token to authorize their account Now the doctor has access Luis

Technologies Luis

Architecture Luis

Database Schema Luis

Data Flow Luis

VM <-> Web Server Scheduling with crontab HTTP Requests with curl File transfer with Azure Command Line Interface Manuel

VM Scripts Workflow Manuel

Website <-> Web Server Asynchronous calls with jQuery|AJAX to upload and download data, and to store/retrieve clusters Three-dimensional output with three.js Manuel

Challenges Migrating initial prototype from MVC 5 to MVC 6 Linux learning curve Three.js and webGL limitations and documentation Funding Manuel