High Resolution Imaging Facility (HRIF) Kent Keyser, Ph.D., Director 205-934-7757 (Office) ktkeyser@uab.edu Shawn Williams, Core Manager 205-934-7403 (Office) tzaron@uab.edu High Resolution Imaging Facility (HRIF) Confocal & Light Microscopy Division The HRIF Confocal & Light Microscopy Division is a cutting-edge facility providing a variety of microscopy services for UAB Scientists. The facility is staffed by experienced technical specialists who are able to offer assistance at each stage of an imaging experiment. To schedule time on a machine or to schedule a training session with an HRIF specialist, visit www.uab.edu/highresolutionimaging. Purpose: High Definition Multichannel Detector for RGB and Far Red Fixed and Live Cell and Tissue Imaging Capabilities: High speed live cell resonance scanner, live cell imaging and tracking, perfect focus for long time lapse. Spectral scanning, FRAP(observation of fluorescence recovery in live cells), 3-D and 2-D imaging, ratiometric imaging for Ca+ flow, colocalization quantification with coeficients in Mander’s and Pearson’s equations. Nikon A1R High Speed Laser Confocal Spectral Imaging Microscope Confocal Core Equipment ImageStreamX Mark II Imaging Flow Cytometer Purpose: Submicron Particle/Exosome Imaging Analysis System Capabilities: Visualizes and measures particles between 10-2000nm (depending on material) in suspension and addresses the needs of a wide variety of applications including: protein aggregation, exosome and microvessicle research, drug delivery systems, characterizes materials under the new EU Definition of Nanomaterials, and is particularly suited to analysis of particles labeled with fluorescent Quantum Dots. Nanosight NS300 Particle Image System Recipient Group 1 Recipient Group 2 Endothelial cells Purpose: High Definition Hybrid Flow Cytometer/Microscope Multichannel RGB Far Red Imaging System Capabilities: Combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insight of microscopy. The system utilizes the same dyes and markers employed in microscopy and flow cytometry and can perform virtually any standard flow cytometry assay with the added value of visual confirmation. Nikon A1R HIGH SPEED Multiphoton Confocal Purpose: High Definition Intravital Multichannel 2-Photon detector for RGB and Far Red Imaging of Live and Fixed Tissue. Capabilities: Live animal (like mice) high speed live cell resonance scanner imaging, cell tracking, and long working distance 25X dipping objective for tissue penetration. Purpose: High Definition Multichannel Quasar detector for RGB and Far Red Imaging Capabilities: 405nm ex pulsed laser for FLIM (detection of fluorescence lifetime decay and ion concentration), FRAP (observation of fluorescence recovery in live cells), FRET (CFP/YFP), study of protein and molecular proximity and distance, and 3-D, 2-D, and spectral imaging. Zeiss LSM 710 Laser Confocal Microscope Additional Confocal Core Equipment: Leica DMRBE-Histological Samples and Fluorescence. Imaging/FLIM/FRAP/FRET 3D capabilities Nikon Diaphot-Fluorescence and Histology Samples Nikon SMZU Stereoscope-disscection/macro samples Cellstar Incubator Thermo-Fisher Cytospin 4 Osteosarcoma Cells Confocal Core Sponsors Institutional Cores Program Comprehensive Cancer Center (Grant No. P30 CA013148) Rheumatic Diseases Core Center (Grant No. P30 AR048311) Veteran’s Administrative Hospital Research Division Comprehensive Arthritis, Musculoskeletal, Bone and Autoimmunity Center