GHS Science CAPT Embedded Task Review Lab Investigation: Enzymes

Your Task A CT apple juice company needs your help to determine how enzymes can be used to produce apple juice from apple sauce. They provide you with two enzymes to see which enzyme will make the most juice. Enzymes: Pectinase Cellulase

Most GHS students used a procedure that looked something like this: Lab Design Most GHS students used a procedure that looked something like this: 1. Place filter paper in a funnel and put funnel in a flask. Funnel with filter paper in it Flask

Lab Design 2. Set up three flasks with funnels. A B C

Lab Design 3. Pour exactly 20 g of apple sauce into each funnel. A B C

Lab Design 4. Add a different enzyme to each funnel. 8 ml of Pectinase 8 ml of Cellulase No enzyme added A B C

Lab Design 5. Let each flask sit for exactly 10 minutes. A B C

Lab Design 6. After 10 minutes, measure how much juice was produced from each flask using a graduated cylinder. Graduated Cylinder Apple Juice Produced Apple Juice Produced Apple Juice Produced A B C

Sample Data Enzyme(s) Flask A Pectinase Flask B Cellulase Flask C No Enzyme Added Amount of Juice Produced 3.0 ml 5.0 ml 0.5 ml

Analysis Questions Directions: On a separate sheet of paper, answer the following questions in complete sentences. Identify the following for this experiment: Problem statement Independent variable Dependent variable

Analysis Questions Identify two variables that were held constant in this experiment. Explain why it is important to hold these variables constant. What was the substrate and the product(s) in this experiment? Why was it important that “flask C” was used? Based on the data, what would your conclusion be for this experiment?

Going Further… Challenge Questions, Set 1 After performing this experiment, you learn from the apple juice company that Pectinase and Cellulase cost different amounts: Pectinase: $.50 per ml Cellulase: $1.00 per ml The apple juice company wants you to be as cost-effective as possible in producing the most amount of juice. Explain to the company which enzyme is most cost-effective (ml/$)?

Going Further… Challenge Questions, Set 2 A group of students are convinced that pectinase is the most cost-effective enzyme to choose to produce apple juice for the company. They think that temperature may affect how much juice pectinase can produce. In a new experiment, they test their hypothesis. Using the same basic design as the original experiment, the students heat apple sauce with pectinase to different temperatures to see which temperature is optimal for producing the most apple juice. The temperatures are: 0°C, 20°C, 40°C, 60°C, 80°C

Going Further… Challenge Questions, Set 2 The results of the students’ new experiment are shown below: 5 ml 10 ml Amount of Apple Juice Produced 0 20 40 60 80 Degrees Celcius

Going Further… Challenge Questions, Set 2 Explain the behavior of pectinase in temperature range 0-20 °C. Explain the behavior of pectinase in temperature range 50-80 °C. What would be an appropriate conclusion for the new experiment?