From: Nerve Growth Factor and Eosinophils in Inflamed Juvenile Conjunctival Nevus Invest. Ophthalmol. Vis. Sci.. 2002;43(6):1850-1856. Figure Legend: Double immunohistochemistry for NGF on eosinophils and mast cells in IJCN conjunctival biopsy specimens. (A) Positive dark brown-red-black double staining for NGF (brown, ) and EG2 (red) demonstrated that most of the eosinophils in the specimen expressed NGF protein. Arrows: colocalization. (B) Positive dark brown-red-black double staining (arrows) for NGF (brown, ) and for AA1 (red) also demonstrated that the majority of mast cells were a source of conjunctival NGF. Magnification: (A, B) ×40. Date of download: 10/18/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.