Day 08 Warm Up- You and your partner will discuss DNA replication vs transcription after watching the 2 videos DNA Replication Transcription/Translation Video

Title: Translation Page: 31 EQ: How does Translation lead to the production of protein? Date: 10/21

Dealing with this part today! Central Dogma Dealing with this part today! DNA mRNA Protein Translation Transcription Nucleus Ribosome

Transcription Review and Translation Intro.

Translation: decoding of an mRNA message into a polypeptide(Protein) chain occurs on a ribosome (OUTSIDE the nucleus)

Translation: mRNA travels outside of the nucleus and connects to the ribosome Nucleus Cytoplasm

2. transfer RNA (tRNA) brings amino acids to the ribosome

3. The ribosome connects the amino acids to form a polypeptide chain (protein) Protein (Polymer) Amino Acid (Monomer)

How does tRNA “know” what amino acid to bring?

Codon GCU CGA Anticodon Codon: three consecutive mRNA bases Ex: GCU Anticodon: three complementary tRNA bases Codon GCU CGA Anticodon

tRNA looks for the codon that pairs with its anticodon Amino Acid tRNA looks for the codon that pairs with its anticodon the ribosome takes the amino acid and attaches it to the polypeptide chain tRNA Anticodon mRNA

ORDER MATTERS! order of DNA bases  order of RNA bases  order of amino acids  what protein is made tRNA Amino acids mRNA

How to use the Codon Chart: Use the left side to find the first letter in the codon Use the top to find the second letter in the codon Use the right side to find the third letter of the codon Go to where ALL three overlap on the chart

DNA = GTA mRNA = CAU

Did you notice?: The codon “AUG” can specify methionine or serve as the “start” codon for protein synthesis.

How to use the Codon Wheel: Begin in the middle with the first letter of the codon Go outward to the second letter in the codon Go outward again to the third letter in the codon. DNA = ATC/ATA mRNA = UAA/UAG

What other codons will tell the ribosome to stop putting amino acids together? What DNA sequences will give you these codes?

Fill in your mRNA strand from you DNA Fill in your tRNA from your mRNA Circle a Codon Use your Codon wheel or Chart to find your amino Acid DNA mRNA A G G C G C G U C U C G A U C tRNA U C C G C G C A G A G C U A G Arginine Arginine Amino Acid Valine Serine Isoleucine ***Remember we use CODONS to read our CODON chart… not the anticodons. Anticodons just bring the correct amino acid.

Codon Bingo! Directions For Playing: If a codon (example: AAG) is called out, you must find the amino acid. If an amino acid (example: serine) is called out, you must find the codon. Hint! When finding a codon, there may be more than one answer…you can count all possible answers on your card!