Anju T. Peters, MD, Atsushi Kato, PhD, Ning Zhang, PhD, David B

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Presentation transcript:

Evidence for altered activity of the IL-6 pathway in chronic rhinosinusitis with nasal polyps Anju T. Peters, MD, Atsushi Kato, PhD, Ning Zhang, PhD, David B. Conley, MD, Lydia Suh, BS, Brian Tancowny, BS, Derek Carter, BS, Tara Carr, MD, Michael Radtke, MD, Kathryn E. Hulse, PhD, Sudarshan Seshadri, PhD, Rakesh Chandra, MD, Leslie C. Grammer, MD, Kathleen E. Harris, BS, Robert Kern, MD, Robert P. Schleimer, PhD Journal of Allergy and Clinical Immunology Volume 125, Issue 2, Pages 397-403.e10 (February 2010) DOI: 10.1016/j.jaci.2009.10.072 Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Assessment of IL-6 and sIL-6R levels in CRS by ELISA. A, IL-6 levels were increased in nasal polyps compared with sinus tissues from controls and CRSsNP. B, sIL-6R levels were increased in the polyp tissue compared to sinus tissue from controls. Levels were marginally increased (P = .06) in CRSwNP compared with CRSsNP. Journal of Allergy and Clinical Immunology 2010 125, 397-403.e10DOI: (10.1016/j.jaci.2009.10.072) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 A, Representative Western blot of p-STAT3 and total STAT3 in nasal polyps and controls. B, Densitometric analysis of p-STAT3 from 4 controls and 6 subjects with CRSwNP shown in A. C, Densitometric analysis of p-STAT3 from 7 controls and 9 subjects with CRSwNP. D, Representative Western blot of p-STAT3 in nasal polyp (n = 3) and inferior turbinate tissue (n = 3) within the same patients with CRSwNP. E,(1) Isotype control antibody staining in control tissue; (2) representative immunostaining for p-STAT3 in control tissue. IT, inferior turbinate; beta-act, beta actin; NS, not significant. Journal of Allergy and Clinical Immunology 2010 125, 397-403.e10DOI: (10.1016/j.jaci.2009.10.072) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 sgp130 levels are increased in polyp tissue compared with sinus tissue from controls by ELISA. Journal of Allergy and Clinical Immunology 2010 125, 397-403.e10DOI: (10.1016/j.jaci.2009.10.072) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Assessment of IL-6 and sIL-6R levels in CRS by ELISA Assessment of IL-6 and sIL-6R levels in CRS by ELISA. A, Levels of IL-6 in nasal lavage samples were not significantly different among the groups. B, sIL-6R was present in the nasal lavage samples, but no differences were observed among the different groups. Journal of Allergy and Clinical Immunology 2010 125, 397-403.e10DOI: (10.1016/j.jaci.2009.10.072) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Secretion of IL-6 by primary nasal epithelial cells cultured from controls, patients with CRSsNP, and patients with CRSwNP at baseline and after stimulation with 25 μg/mL dsRNA. A, IL-6 secretion by epithelial cells from the inferior turbinate. B, IL-6 secretion by epithelial cells collected from the uncinate process. Journal of Allergy and Clinical Immunology 2010 125, 397-403.e10DOI: (10.1016/j.jaci.2009.10.072) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Levels of sgp130 by ELISA assessed in nasal lavage showed no significant differences among the groups. Journal of Allergy and Clinical Immunology 2010 125, 397-403.e10DOI: (10.1016/j.jaci.2009.10.072) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Assessment of IL-17 A, E, and F levels by ELISA in tissue extracts from controls (IL-17A, n = 17; IL-17E, n = 15; IL-17F, n = 16), CRSsNP (IL-17A, n = 20; IL-17E, n = 18; IL-17F, n = 17), and CRSwNP (IL-17A, n = 20; IL-17E, n = 12; IL-17F, n = 14). Journal of Allergy and Clinical Immunology 2010 125, 397-403.e10DOI: (10.1016/j.jaci.2009.10.072) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions