Presented By: Sarah A. Gregory

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Presentation transcript:

Light Exposure Increases Dissolved Organic Carbon Uptake in Wyoming Rivers Presented By: Sarah A. Gregory Co-Authors: Erin R. Hotchkiss & Robert O. Hall, University of Wyoming

Acknowledgements Special Thanks to: NSF REU Program UW EPSCoR Program ENR Haub Research and Creative Activities Grant Honors Program NSF Big Rivers Project Summer 2010 In Collaboration with Utah State University, University of Notre Dame, and Cary Institute of Ecosystem Studies

Inland Waters Assumed to be Passive Carbon Transporters

Past Assumption: Inland Waters Are Pipes

Inland Waters Process More than ½ of Carbon From Land Initial Model New Model

Dissolved Organic Carbon Represents a Majority of Total Organic Carbon In Inland Waters Known as DOC <0.45μm DOC enters inland waters from: External Loading = Terrestrial Internal Processes = Algal

Available DOC can be consumed by bacteria Availability based on structure of DOC Highly available is readily consumed by microbes DOC source is linked to the availability of DOC Due to structure of DOC from source

Light Exposure Increases DOC Availability UV changes molecular structure High molecular weight is less available Low Molecular Weight High Molecular Weight

Research Questions What is the effect of light exposure on uptake? What is the interaction between uptake and spectral absorbance (indicator of molecular weight)?

We Added Leaves to Water to Leach Terrestrial DOC Soil Rock Scrubbings

Light and Dark Treatments to Quantify the Changes of DOC Availability

Biological Assays are Used to Calculate Bacterial Consumption of DOC Filtered Water + Bacterial Inoculum Change in DOC from start to end is the total DOC consumed by bacteria Bacterial Consumption=Uptake=K (d-1)=(-ln(docend-docstart)) / time

Light Exposure Increases Uptake -Laramie River

Light Exposure Increases Uptake -Snake River 2 Way ANOVA Statistically Significant

Biofilm River Water Leaves Changes in Molecular Structure Due to Light is Source Dependent Biofilm River Water K (d-1) Light Dark Leaves Soil Low (250): High MW (365)

Summary Light exposure increased bacterial uptake of DOC Changes in DOC structure after light exposure depends on source

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