MIXING AND AERATION (SCALE-UP)

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Presentation transcript:

MIXING AND AERATION (SCALE-UP)

INTRODUCTION Problem of increased scale revolve around mixing and gas exchange. Aim at achieving maximum movement of liquid and minimum shear stress. Additives may be added like Pluronic F68, CMC, PVD to avoid shear.

CULTURE BAGS Plastic Bags- Gas permeable. Agitated on a flat rocking platform Capacity range from 0.5L to 10L.

AIR LIFT FERMENTORS Here a cyclic flow of medium (caused by air provided at base) allows both agitation and aeration. Circulation time in loop of 45m may be near 120 seconds. Fermenters of upto 20,000L capacities have been used.

Riser tube Downcomer tube Medium with lower density Medium with higher density Airlift fermenter

BelloCell AERATOR CULTURE Compartment having cells Porous membrane Bellows medium compartment

PERFUSED SUSPENSION CULTURE 1.Cells retained at high concentration. 2.Medium perfused through hollow fibers. 3.External regulation.

FLUIDIZED BED REACTORS 1.Porous Microcarriers made of Ceramics. 2.Beads have Low density. 3.Sedimentation rate of Beads= Medium flow rate 4.Cells grow in the interstices of the bead matrix.

THANK YOU