ERT 445 FINAL YEAR PROJECT 1 METHODOLOGY Dr. Syazni Zainul Kamal Free Powerpoint Templates

INTRODUCTION Now you are actually talking about what you are proposing. be sure that this follows naturally from the Introduction in which you should have set up and highlighted some critical issue that needs to be resolved. tell the reader how you would resolve this issue.

PURPOSE This section will provides a written description of the specific actions, plan, or strategy you will take to answer your research questions.

METHODOLOGY HOW YOU INVESTIGATE THE QUESTION? WHAT WILL YOU DO? HOW YOU WILL DO IT?

CONTENTS This section should describe the proposed method for the experiment, including Research Framework (Overall Research Work) List of Equipments and Materials (that will be used) Procedure (that will be followed)

RESEARCH FRAMEWORK/FLOW CHART Overall view of steps/flow in your research Use mind map Must have continuous flow

Example Sampling Bacteria Source Pre - Treatment Testing Using RCM Medium in Serum Bottle Evaluation H2 Potential Active Inoculum Stock Culture Optimization Studies Inoculum Development pH Characterization of Agriculture Waste Kinetic Study Dark Fermentation No 1 2 Yes Example

Equipment and materials In this subsection of the method section you describe any equipment and materials/chemicals in your research study. The following information should be included: General description of the apparatus or instruments. Reference indicating where equiments can be obtained.

Material Brand 2,2-diphenyl-1-picrylhydrazyl Sigma, Malaysia Ethanol Merck, Malaysia Ethyl acetate Acetone Equipment Location Beaker School of Bioprocess, UniMAP Test tube Incubator Grinder UV-VIS Spectrophotometer

PROCEDURE In this subsection of the methodology section of your proposal, you carefully describe how your study will be executed. The following information should be included in the procedure section: A description of the design if it was not previously described. A detailed step-by-step description of how the study will be executed.

PROCEDURE list down all scopes with details description -material/sample preparation, design work, simulation/modelling work, development or fabrication work, characterization and optimization -should specify the variables/parameters (independent and dependent) that will be used in the experiment with the min and max range that will be run by DOE -Determine how many of replicate / control

5.3 Procedure 5.3.1 Plant collection and sample preparation The plant of Stachytarpheta indica will be collected from Kampung Wang Tepus, Jitra, Kedah. The whole plant collected will be washed thoroughly under running tap water to remove the dust and soil. Then, the plant will undergo drying process by using oven at 50°C overnight. 5.3.2 Plant extraction process Explain in detail the process 5.3.3 Antibacterial assay 5.3.4 Phytochemical analysis

RUBRIC NO CRITERIA EXEMPLARY (4) PROFICIENT (3) ACCEPTABLE (2) UNACCEPTABLE (1) 1 Rational of Techniques Used -All techniques used during the experiment very well explained. -All techniques used during the experiment were explained. -Some techniques used during the experiment were not explained clearly. -Some techniques used during the experiment were not explained. 2 Understanding of Concept and Principle -Demonstrate an in-depth understanding of concept and principles of each method. -Demonstrate an understanding of concept and principles of each method. -Demonstrate an inadequate understanding of concept and principles of each method. -Unable to demonstrate understanding of methodology concept. 3 Sequence -Actions well presented in a logical sequence. -Actions presented in a logical sequence. -Some of the actions are not presented in a logical sequence. -Actions were not presented in a logical sequence. 4 Materials -All the materials used were included and clearly described. -The materials used were included generally. -Some of the materials used were included. -None of the materials used were included. 5 Grammar and Sentence Structure -No errors in spelling and grammar. Appropriate tenses were used. -Seldom errors in spelling and grammar. Appropriate tenses were used. -Frequent errors in spelling and grammar. Appropriate tenses were used partly. -Abundance of errors in spelling and grammar. No appropriate tenses were used.

EXPECTED RESULT WHAT DO YOU EXPECT TO FIND? Based on the present researches, the zone of inhibition obtained in the disc diffusion test should be more than 14mm to shown the plant extract has the ability to inhibit the bacteria growth. The MIC obtained should be less than 1000ug/ml in order for the plant extract to possess antibacterial activities. The plant extract should give a stronger antibacterial effect to gram-positive bacteria than gram-negative bacteria.

Any questions?

Progress report submission due date NEW DATE OF SUBMISSION Progress report submission due date Introduction, Problem statement, research objective, literature review, references of proposal report (2 copies) 30 Sept 2016 Methodology (2 copies) 14 Oct 2016 Finalized proposal report (4 copies) 19 Oct 2016 Proposal defense (slide presentation) 31Oct-4Nov 2016 (after mid sem break) Corrected proposal (2 copies) 11Nov 2016

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