Which sample preparation? Identification/ quantitation?

Slides:

Advertisements

Similar presentations

Chemistry Dept, UCONN Identification of Drug Metabolites via Mass Spectrometry Besnik Bajrami Chem 395.

Advertisements

Agilent 6410 Triple Quad LC/MS Software

Gas Chromatography There an be many parts to a gas chromatography system but the basic components include: An injection system. A column (controllable.

The Proteomics Core at Wayne State University

1 1. Introduction H: High P : Performance (Pressure) L : Liquid C : Chromatography GC : Gas chromatography TLC: Thin layer chromatography IC : Ion chromatography.

Pesticide screening LC-QTOF, Agilent. National Food Institute, Technical University of Denmark Disposition National Food Institute –EURL –NRL –Personale.

High Performance Liquid Chromatography. HPLC originally refered to: High Pressure Liquid Chromatography currently refers to: High Precision Liquid Chromatography.

Cell Separation Using Magnetic Beads

Metabolomics DNA RNA Protein Biochemicals (Metabolites) Genomics – 25,000 Genes Transcriptomics – 100,000 Transcripts Metabolomics – 2,800 Compounds Proteomics.

Yoona Kim University of California, San Diego UCSD Mass Spectrometry Journal Club 12/03/10.

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL Label Free Quantitative Proteomics “biomarker discovery”

An introduction to chromatography. To identify the compounds of a mixture = qualitative analysis To quantify these compounds To retrieve the separated.

FIGURE 5. Plot of peptide charge state ratios. Quality Control Concept Figure 6 shows a concept for the implementation of quality control as system suitability.

Evaluated Reference MS/MS Spectra Libraries Current and Future NIST Programs.

Pn. Suryati Bt. Syafri 2009 High Performance Liquid Chromatography (HPLC)

Tryptic digestion Proteomics Workflow for Gel-based and LC-coupled Mass Spectrometry Protein or peptide pre-fractionation is a prerequisite for the reduction.

Analysis of Hot Spring Microbial Mat

Advanced Electrophoresis Solutions Ltd

2007 GeneSpring MS GeneSpring for Metabolite BioMarker Analysis using Mass Spectrometry data Agilent Q-TOF VIP Visit Jan 16-17, 2007 Santa Clara, CA Thon.

High Performance Liquid Chromatography (HPLC) Jeannette Comeau CHEE April 2004.

What is analytical chemistry? 5 subdisciplines of chemistry Some definitions In every day life… Analytical chemist versus chemical analyst.

GSAT501 - proteomics Name, home-town Students – previous lab experience –Lab you hope to end up in? Teachers – what is your current project.

ProteoRed Multicentric Experiment 5: Intensity-based Label-free quantification results Kerman Aloria (University of the Basque Country, UPV/EHU) WG1-WG2.

Additional file 1 1.1Workflow of large-scale proteomic analysis of normal human kidney glomerulus 1.2Detailed procedure of LC-MS/MS analysis Additional.

Laboratori de Proteòmica Vall d’Hebron Institut d’Oncologia Francesc Canals Madrid 28/08/2012 Human Proteome Project – CHR16 SRM Analysis of selected proteins.

Motion Vocabulary. The act or process of changing position or place. 1.

ProteoRed WG1-WG2 Meeting Salamanca, March,16th 2010 PME5: Quantitative LC-MS differential analysis F. Canals.

QUANTITATIVE ANALYSIS Determining amount of ions present in samples.

LECTURE 2 Discrete Quantitative Data A simple frequency distribution, frequency bar char can be used to organize the discrete quantitative variable. Example.

Chapter 28 High Performance Liquid Chromatography.

Dr Saleha Shamsudin. 1.INTRODUCTION Topics to be covered: 1.1 INTRODUCTION TO METHODS IN ANALYTICAL CHEMISTRY 1.2 STEPS IN QUANTITATIVE ANALYSIS.

Proteomics Informatics (BMSC-GA 4437) Instructor David Fenyö Contact information

ProteoRed WG1-WG2 Meeting Salamanca, March,16th 2010 PME5: Quantitative LC-MS differential analysis F. Canals.

Salamanca, March 16th 2010 Participants: Laboratori de Proteomica-HUVH Servicio de Proteómica-CNB-CSIC Participants: Laboratori de Proteomica-HUVH Servicio.

4th Grade Common Core Math Practice and Solutions for 4th Grade Common Core Math Standard 4.NF.7 Multiple Choice Set #1 without Answers Multiple Choice.

What is proteomics? Richard Mbasu and Ben Richards.

The world leader in serving science For Research Use Only. Not for use in diagnostic procedures Quantitative Analysis of 4 Immunosuppressant Drugs in Whole.

CU-Boulder Central Analytical Lab Mass Spectrometry Core Facility JSCBB C1B90 Jeremy Balsbaugh & Thomas Lee.

Using Scaffold OHRI Proteomics Core Facility. This presentation is intended for Core Facility internal training purposes only.

In-vitro metabolite profiling of by LC-MS/MS Tejaswini Kailas Jadhav MS (Pharmaceutical Analysis) NIPER-A.

High Performance Liquid Chromatography

Acids, Bases, and pH.

Richard Mbasu and Ben Richards

Two Dimensional Gel Electrophoresis (2DE) of extracellular protein of Talaromyces verruculosus SGMNPf3 (a) Standard (b) sample study.

Department of Medical and Clinical Biophysics Faculty of Medicine Pavol Jozef Šafárik University in Košice, Slovakia Expertise in: Mass spectrometry based.

Sample Preparation Enzymatic Digestion (Trypsin) + Fractionation.

Volume 4, Issue 6, Pages e4 (June 2017)

Speed Formula - Waves.

2 Dimensional Gel Electrophoresis

HPLC Equipment : Detectors

EPP Peak #1 Extraction 1 Extraction 2 Extraction 3 Gel 1 Gel 2

Try this while you wait:

MCB test 2 Review M. Alex Miranda 11/5/16.

بسم الله الرحمن الرحیم.

Volume 4, Issue 6, Pages e4 (June 2017)

Beer's- Lambert Law and Standard Curves of concentrations

Protein Identification Using Tandem Mass Spectrometry

Shotgun Proteomics in Neuroscience

Stability and variation of metrics over a range of sample injection amounts. Stability and variation of metrics over a range of sample injection amounts.

C.2.10 Sample Questions.

C.2.8 Sample Questions.

C.2.8 Sample Questions.

Sample preparation Protein and peptide separation techniques Karel Bezstarosti (Proteomics Center, Erasmus MC)

Phosphorylated tyrosine residues in FGFR3, FGFR3(K650E), FGFR3-TACC3, and FGFR3(K650E)-TACC3 identified by mass spectrometry analysis. Phosphorylated tyrosine.

Solution Free Body Diagram.

Presentation transcript:

Which sample preparation? Identification/ quantitation? 4 h gradient 1h gradient Protocol 2 Protocol 5 Protocol 4 Protocol 3 Protocol 1 Which sample type? Cells/tissues Body fluids IP/AP/purified proteins/gel slices Fractionation? YES NO Which sample preparation? In-gel In-solution MARS-14 depletion Length of LC method? UHR-QTOF (maXis 4G+) MS instrument ID+LFQ Identification/ quantitation? ID Ion trap (amaZon Speed) MaxQuant Data analysis Mascot Analysis C Analysis A Choice of analysis Analysis B