Analysis of hydrogen peroxide field samples by HPLC/FD and HPLC/ED in DC mode Megan Tarvin, Bruce McCord, Kelly Mount, Mark L. Miller Forensic Science International Volume 209, Issue 1, Pages 166-172 (June 2011) DOI: 10.1016/j.forsciint.2011.01.024 Copyright © 2011 Terms and Conditions
Fig. 1 Analysis of a plastic remnant by HPLC/ED in DC mode. Conditions: Dionex CarboPac PA10 analytical column, isocratic elution with 150mM NaOAc at pH 10.5, flow rate 1mL/min, 100μL injection, T=45°C, DC mode with E=+0.4V. Peak 1, hydrogen peroxide, Rt 1.902min. Forensic Science International 2011 209, 166-172DOI: (10.1016/j.forsciint.2011.01.024) Copyright © 2011 Terms and Conditions
Fig. 2 Analysis of a plastic remnant by HPLC/FD. Conditions: Dionex C-18 analytical column, isocratic elution with 1×10−3M H2SO4/1×10−4M EDTA at flow rate 0.6mL/min, 100μL injection, reagent 8μM hemin/80μM POPHA in ammonia buffer pH 9.5 at flow rate 0.2mL/min, post-column reactor 2m, T=25°C, base 0.1M NaOH at flow rate 0.2mL/min, λex=320nm, λem=405nm. Peak 1, hydrogen peroxide, Rt 5.195min. Forensic Science International 2011 209, 166-172DOI: (10.1016/j.forsciint.2011.01.024) Copyright © 2011 Terms and Conditions
Fig. 3 Structures of TATP (left) and HMTD (right). Concentrated hydrogen peroxide is a precursor in the manufacturing of both of these explosives. Forensic Science International 2011 209, 166-172DOI: (10.1016/j.forsciint.2011.01.024) Copyright © 2011 Terms and Conditions
Fig. 4 Analysis of post-blast residue of TATP by HPLC/ED in DC mode. Conditions: Dionex CarboPac PA10 analytical column, isocratic elution with 150mM NaOAc pH 10.5, flow rate 1mL/min, 100μL injection, T=45°C, DC mode with E=+0.4V. Peak 1, hydrogen peroxide, Rt 1.995min. Forensic Science International 2011 209, 166-172DOI: (10.1016/j.forsciint.2011.01.024) Copyright © 2011 Terms and Conditions
Fig. 5 Analysis of post-burn residue of HMTD by HPLC/FD. Conditions: Dionex C-18 analytical column, isocratic elution with 1×10−3M H2SO4/1×10−4M EDTA at flow rate 0.6mL/min, 100μL injection, reagent 8μM hemin/80μM POPHA in ammonia buffer pH 9.5 at flow rate 0.2mL/min, post-column reactor 2m, T=25°C, base 0.1M NaOH at flow rate 0.2mL/min, λex=320nm, λem=405nm. Peak 1, hydrogen peroxide, Rt 5.217min. Forensic Science International 2011 209, 166-172DOI: (10.1016/j.forsciint.2011.01.024) Copyright © 2011 Terms and Conditions
Fig. 6 Analysis of post-burn residue of HMTD by HPLC/ED in DC mode. Conditions: Dionex CarboPac PA10 analytical column, isocratic elution with 150mM NaOAc pH 10.5, flow rate 1mL/min, 100μL injection, T=45°C, DC mode with E=+0.4V. Peak 1, unknown, Rt 1.526min; peak 2, hydrogen peroxide, Rt 1.927min; peak 3, unknown, Rt 2.984min. The unknown peaks may correspond to combustion products of HMTD or to materials remaining from the synthesis of the material. Forensic Science International 2011 209, 166-172DOI: (10.1016/j.forsciint.2011.01.024) Copyright © 2011 Terms and Conditions
Fig. 7 Analysis of post-burn residue of HMTD by HPLC/FD. Conditions: Dionex C-18 analytical column, isocratic elution with 1×10−3M H2SO4/1×10−4M EDTA at flow rate 0.6mL/min, 100μL injection, reagent 8μM hemin/80μM POPHA in ammonia buffer pH 9.5 at flow rate 0.2mL/min, post-column reactor 2m, T=25°C, base 0.1M NaOH at flow rate 0.2mL/min, λex=320nm, λem=405nm. Peak 1, hydrogen peroxide, Rt 5.242min. Unlike in the analysis of intact HMTD, no extraneous peaks are noted in the HPLC/FD chromatogram of post-blast HMTD. Forensic Science International 2011 209, 166-172DOI: (10.1016/j.forsciint.2011.01.024) Copyright © 2011 Terms and Conditions