Fig. 1 Increased MIR155HG expression correlates with glioma grade and mesenchymal transition and confers a poor prognosis in GBM patients. (A) The level.

Slides:

Advertisements

Similar presentations

Fig. 3. Plot of overall survival by adjuvant therapy for patient subgroups. (A) Patients with M0 disease (n = 411). (B) Patients receiving 36 Gy craniospinal.

Advertisements

Volume 342, Issue 1, Pages (January 2014)

Fig. 1 GBM TIMs expand to inhibit vaccine-induced T-cell mediated tumor cytolysis via PD-1/PD-L1 regulatory pathway. (A, D) CD163, DAPI, (B, E) PD-L1,

Bufalin Inhibits the Differentiation and Proliferation of Cancer Stem Cells Derived from Primary Osteosarcoma Cells through Mir-148a Cell Physiol Biochem.

MicroRNA-101 Inhibits Growth, Proliferation and Migration and Induces Apoptosis of Breast Cancer Cells by Targeting Sex-Determining Region Y-Box 2 Cell.

Fig. 1. Immunostaining for AhR in non-neoplastic urothelium ( A ), superficial UC ( B ) and invasive UC ( C ). ( A ) Weak cytoplasmic staining in the.

Fig. 6. Knockdown of TCF-4 inhibits TCTP-induced glioma cell proliferation in vitro and in vivo. (A) TCF-4 was knocked down in U-251/TCTP and H-4/TCTP.

The Long Non-Coding RNA XIST Interacted with MiR-124 to Modulate Bladder Cancer Growth, Invasion and Migration by Targeting Androgen Receptor (AR) Cell.

Fig. 4. Effect of FTY720 on brain tumor stem cell (BTSC) invasiveness

Figure 1 miRNA expression in multiple sclerosis lesions

Cell Physiol Biochem 2017;44:1867– DOI: /

Elevated FOXC2 Expression Promotes Invasion of HCC Cell Lines and is Associated with Poor Prognosis in Hepatocellular Carcinoma Cell Physiol Biochem 2017;44:99–109.

MicroRNA-558 regulates the expression of cyclooxygenase-2 and IL-1β-induced catabolic effects in human articular chondrocytes S.J. Park, E.J. Cheon,

Upregulation of miR-142-3p Improves Drug Sensitivity of Acute Myelogenous Leukemia through Reducing P-Glycoprotein and Repressing Autophagy by Targeting.

Cyclin-Dependent Kinase 2 Promotes Tumor Proliferation and Induces Radio Resistance in Glioblastoma Jia Wang, Tong Yang, Gaofeng Xu, Hao Liu, Chunying.

Cell Physiol Biochem 2015;36: DOI: /

MEF Promotes Stemness in the Pathogenesis of Gliomas

IMP-3 Promotes Migration and Invasion of Melanoma Cells by Modulating the Expression of HMGA2 and Predicts Poor Prognosis in Melanoma Yi-Shuan Sheen,

WT1 Promotes Invasion of NSCLC via Suppression of CDH1

Volume 133, Issue 2, Pages (August 2007)

Strong radioprotective FGF1 signaling down-regulates proliferative and metastatic capabilities of the angiosarcoma cell line, ISOS-1, through the dual.

Sp1 Suppresses miR-3178 to Promote the Metastasis Invasion Cascade via Upregulation of TRIOBP Hui Wang, Kai Li, Yu Mei, Xuemei Huang, Zhenglin Li, Qingzhu.

Snail Enhances Glycolysis in the Epithelial-Mesenchymal Transition Process by Targeting FBP1 in Gastric Cancer Cell Physiol Biochem 2017;43:31–38 - DOI: /

Up-Regulation of RFC3 Promotes Triple Negative Breast Cancer Metastasis and is Associated With Poor Prognosis Via EMT Zhen-Yu He, San-Gang Wu, Fang Peng,

SDHB deficiency promotes TGFβ-mediated invasion and metastasis of colorectal cancer through transcriptional repression complex SNAIL1-SMAD3/4 Haiyu Wang,

MicroRNA-489 Plays an Anti-Metastatic Role in Human Hepatocellular Carcinoma by Targeting Matrix Metalloproteinase-7 Yixiong Lin, Jianjun Liu, Yuqi Huang,

UHRF1 is regulated by miR-9 in colorectal cancer

MicroRNA-381 Represses ID1 and is Deregulated in Lung Adenocarcinoma

MicroRNA-558 regulates the expression of cyclooxygenase-2 and IL-1β-induced catabolic effects in human articular chondrocytes S.J. Park, E.J. Cheon,

Xiaqin Sun, Yu Wu, Mingxue Gu, Yan Zhang Cell Reports

Molecular Therapy - Nucleic Acids

Volume 140, Issue 1, Pages (January 2011)

Volume 19, Issue 3, Pages (March 2017)

Volume 20, Issue 2, Pages (February 2018)

Uc.454 Inhibited Growth by Targeting Heat Shock Protein Family A Member 12B in Non- Small-Cell Lung Cancer Jun Zhou, Chenghai Wang, Weijuan Gong, Yandan.

Relative mRNA expression level (/J3T-1)

Volume 152, Issue 1, Pages (January 2019)

Volume 22, Issue 10, Pages (October 2014)

Volume 16, Issue 6, Pages (August 2016)

Molecular Therapy - Nucleic Acids

Volume 25, Issue 3, Pages (March 2017)

MicroRNA-101 Exerts Tumor-Suppressive Functions in Non-small Cell Lung Cancer through Directly Targeting Enhancer of Zeste Homolog 2 Ji-guang Zhang,

Molecular Therapy - Nucleic Acids

Inhibition of KLF4 by Statins Reverses Adriamycin-Induced Metastasis and Cancer Stemness in Osteosarcoma Cells Yangling Li, Miao Xian, Bo Yang, Meidan.

Volume 23, Issue 10, Pages (October 2016)

Fig. 5. GUCD1 was a direct target of miR-370 in HCC

MiR-135b Stimulates Osteosarcoma Recurrence and Lung Metastasis via Notch and Wnt/β-Catenin Signaling Hua Jin, Song Luo, Yun Wang, Chang Liu, Zhenghao.

miR-124 Inhibits Lung Tumorigenesis Induced by K-ras Mutation and NNK

Promotion Effects of miR-375 on the Osteogenic Differentiation of Human Adipose- Derived Mesenchymal Stem Cells Si Chen, Yunfei Zheng, Shan Zhang, Lingfei.

Lu Zheng, Nan You, Xiaobing Huang, Huiying Gu, Ke Wu, Na Mi, Jing Li

Kun-Peng Zhu, Xiao-Long Ma, Chun-Lin Zhang Molecular Therapy

Volume 19, Issue 8, Pages (August 2011)

Figure 1. RSPO3 expression is upregulated in bladder cancer

Figure 1. LOC is highly expressed in NPC and predicts unfavorable prognosis. (A) Differential gene expression ... Figure 1. LOC is highly expressed.

MicroRNA-381 Represses ID1 and is Deregulated in Lung Adenocarcinoma

Volume 26, Issue 8, Pages (August 2018)

Molecular Therapy - Nucleic Acids

Long Noncoding RNA BC as a Novel Therapeutic Target for Colorectal Cancer that Suppresses Metastasis by Upregulating TIMP3 Jiaxin Lin, Xin Tan,

MELK Promotes Melanoma Growth by Stimulating the NF-κB Pathway

Negative Regulation of Tumor Suppressor p53 by MicroRNA miR-504

Volume 6, Issue 3, Pages (March 2016)

MiR-409 Inhibits Human Non-Small-Cell Lung Cancer Progression by Directly Targeting SPIN1 Qi Song, Quanbo Ji, Jingbo Xiao, Fang Li, Lingxiong Wang, Yin.

The lncRNA PDIA3P Interacts with miR-185-5p to Modulate Oral Squamous Cell Carcinoma Progression by Targeting Cyclin D2 Cheng-Cao Sun, Ling Zhang, Guang.

Volume 23, Issue 4, Pages (April 2015)

MiR-431-5p directly targets RAF1 and is negatively correlated to RAF1 expression (A) The predicted binding sites of miR-431-5p and RAF1 3′-UTR, and mutant.

Volume 22, Issue 9, Pages (September 2014)

The Expression of MicroRNA-598 Inhibits Ovarian Cancer Cell Proliferation and Metastasis by Targeting URI Feng Xing, Shuo Wang, Jianhong Zhou Molecular.

Volume 24, Issue 10, Pages (October 2016)

Volume 26, Issue 9, Pages (September 2018)

Volume 23, Issue 4, Pages (April 2015)

Presentation transcript:

Fig. 1 Increased MIR155HG expression correlates with glioma grade and mesenchymal transition and confers a poor prognosis in GBM patients. (A) The level of MIR155HG was analyzed in glioma tissues of the CGGA and REMBRANDT glioma datasets. (B) Kaplan–Meier survival curves for MIR155HG expression in GBM tissues of the CGGA and REMBRANDT datasets. (C) In silico analysis of MIR155HG mRNA expression and the correlation with its associated genes in glioma of CGGA datasets. A heatmap of relative expression of MIR155HG differentially expressed genes in glioma tissues sorted by level of MIR155HG expression. (D) GSEA analysis showed that there was enriched expression of mesenchymal transition–associated genes in high MIR155HG expression samples of GBM. (E) The expression of MIR155HG in 10 primary GBM samples is higher than that in 10 normal brain tissues. (F) The expressions of POSTN, MMP11, and FN1 are increased in GBM samples with high MIR155HG expression compared with those with low MIR155HG expression. (G) Decreased expression of MIR155HG regulated the expression of common mesenchymal transition–associated markers in U87 and primary GBM cells. From: Blocking MIR155HG/miR-155 axis inhibits mesenchymal transition in glioma Neuro Oncol. 2017;19(9):1195-1205. doi:10.1093/neuonc/nox017 Neuro Oncol | © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

Fig. 5 NSC141562 is a potent inhibitor of the MIR155HG/miR-155 axis Fig. 5 NSC141562 is a potent inhibitor of the MIR155HG/miR-155 axis. (A) MiR-155-5p relative expression assayed using RT-PCR after small-molecule inhibitor treatment at a half-maximal inhibitory concentration dose in U87 and primary GBM cells. (B and C) The results of wound healing assay (scale bar: 100 μm) and transwell assay (scale bar: 200 μm) showed that NSC141562 suppressed glioma cell migration and invasion. (D) Western blot assays revealed that NSC141562 significantly increased PCDH9/7 expression and decreased the expression of mesenchymal transition–associated markers. *P < .05, **P < .01, ***P < .001. From: Blocking MIR155HG/miR-155 axis inhibits mesenchymal transition in glioma Neuro Oncol. 2017;19(9):1195-1205. doi:10.1093/neuonc/nox017 Neuro Oncol | © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

Fig. 2 MIR155HG knockdown inhibits glioma malignant phenotypes Fig. 2 MIR155HG knockdown inhibits glioma malignant phenotypes. (A) Wound healing assay of glioma cells transfected with control or siMIR155HG. The wound was photographed at different time points and wounded gaps were analyzed by measuring the distance of migrating cells for 3 different areas for each wound (scale bar: 100 μm). (B) SiMIR155HG inhibited the invasion of U87 and primary GBM cells. Cells were examined for cell invasion in 24-well plates with transwell chambers (scale bar: 200 μm). The invasiveness of glioma cells was attenuated after the decreased expression of MIR155HG. (C) Plot of the Fluc activity by bioluminescence imaging for intracranial tumors. (D) Representative images of mice implanted with intracranial tumors on days 3, 7, 14, 21, 28, and 35. (E) Overall survival of nude mice was determined by Kaplan–Meier survival curves, and the log-rank test was used to assess the statistical significance of the differences. **P < .01. From: Blocking MIR155HG/miR-155 axis inhibits mesenchymal transition in glioma Neuro Oncol. 2017;19(9):1195-1205. doi:10.1093/neuonc/nox017 Neuro Oncol | © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

Fig. 3 MiR-155-5p and miR-155-3p are functionally relevant effectors of MIR155HG-induced cell proliferation and invasion. (A) MIR155HG knockdown remarkably reduced miR-155-5p and miR-155-3p expression in U87 and primary GBM cells. (B) Effect of anti–miR-155-5p and anti–miR-155-3p on cell migration (scale bar: 100 μm). (C) Cell invasion ability was analyzed after differential treatment via transwell assay (scale bar: 200 μm). (D and E) Overexpression of miR-155-5p and miR-155-3p reversed the siMIR155HG-induced inhibition of cell migration (scale bar: 100 μm) and invasion (scale bar: 200 μm).*P < .05, **P < .01, ***P < .001. From: Blocking MIR155HG/miR-155 axis inhibits mesenchymal transition in glioma Neuro Oncol. 2017;19(9):1195-1205. doi:10.1093/neuonc/nox017 Neuro Oncol | © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

Fig. 4 PCDH9 and PCDH7 are the downstream target genes of miR-155 and function as tumor suppressors by inhibiting the Wnt/β-catenin signaling pathway. (A) Putative binding sites of miR-155-5p within the PCDH9 3ʹ UTR predicted by TargetScan, and putative binding sites of miR-155-3p within the PCDH7 3ʹ UTR predicted by microRNA. (B) Western blot analysis showed that PCDH9 and PCDH7 expression were upregulated after miR-155 expression was inhibited. (C) MiR-155-5p downregulated luciferase activities controlled by wild-type PCDH9 3ʹ UTR but did not affect luciferase activity controlled by mutant PCDH9 3ʹ UTR. And miR-155-3p downregulated luciferase activities controlled by wild-type PCDH7 3ʹ UTR but did not affect luciferase activity controlled by mutant PCDH7 3ʹ UTR. (D) Overexpression of PCDH9 and PCDH7 downregulated the expression of β-catenin and cyclin D1 and upregulated pβ-catenin expression. *P < .05, **P < .01, ***P < .001. From: Blocking MIR155HG/miR-155 axis inhibits mesenchymal transition in glioma Neuro Oncol. 2017;19(9):1195-1205. doi:10.1093/neuonc/nox017 Neuro Oncol | © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com