SEMINAR 1. Title : Direct measurements of inter-membrane forces using Surface Forces Apparatus (SFA): Role of membrane protein concentrations and lipid.

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SEMINAR 1. Title : Direct measurements of inter-membrane forces using Surface Forces Apparatus (SFA): Role of membrane protein concentrations and lipid compositions 2. Speaker : Dong Woog Lee ( UNIST ) 3. Time : 16:00 – 17:00, Thursday, November 17, 2016 4. Place : e+ Lecture Hall (room 83188), 2nd Research Building, Sungkyunkwan University 5. Summary : Interaction forces between lipid membranes (bilayers) were directly measured using a Surface Forces Apparatus (SFA). This talk contains two different but closely related works. In the first part of this talk, the effects of Myelin Basic Protein (MBP, an inter-membrane protein) concentrations and lipid compositions on the inter-membrane adhesion forces will be discussed. As model lipid membranes, two different compositions of lipid mixtures are used: (i) the composition of a healthy myelin, and (ii) the composition of a myelin suffering from Experimental Allergic Encephalomyelitis (EAE), which is an animal model for Multiple Sclerosis. In the second part of the talk, a pressure induced hemifusion event of two supported model lipid bilayers have been imaged using a Fluorescence-SFA. Using this technique, evolution of the membrane thickness and the rearrangement of lipid rafts are monitored simultaneously. Also, the effects of applied pressure on the rate of bilayer hemifusion is investigated. 6 Background : Education 2008. 09-2014. 01 Ph.D., Chemical Engineering, UC Santa Barbara 2002. 03-2008. 02 B.S., Chemical and Biomolecular Engineering, KAIST Work 2016. 02-Current Assistant Prof., Energy and Chemical Engineering, UNIST 2014. 02-2016. 02 Postdoc, Chemical Engineering, UC Santa Barbara 7. Questions : (☏ 031-299-4115)