RESULTS CONCLUSIONS PROCESSING FRESH MUSSELS (M. GALLOPROVINCIALIS)

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RESULTS CONCLUSIONS PROCESSING FRESH MUSSELS (M. GALLOPROVINCIALIS) 46th WEFTA Split, Croatia /12-14 Oct 2016 PROCESSING FRESH MUSSELS (M. GALLOPROVINCIALIS) BY SOUS VIDE TECHNOLOGY: EFFECT ON THE MICROBIOLOGICAL CHARACTERISTICS Bongiorno T.a*, Tulli F. b, Comi G. b, Sensidoni A. b, Iacumin L. b a CRITA S.c.a.r.l. Via Pozzuolo, 324, 33100 Udine, Italy bDepartment of Agricultural Food Environmental and Animal Sciences, Udine University, Italy *Corresponding author: tiziana.bongiorno@uniud.it INTRODUCTION MATERIALS AND METHODS Sous-vide cook-chilled (SVCC) refers to food that is subjected to vacuum-pack and then mild heat treated under controlled conditions (t and T) and subsequently rapidly cooled and stored at chilled conditions until heated before serving (Rhodehamel, 1992; Hansen et al., 1995). Few studies reported the application of this process for aquaculture products (Espinosa et al., 2016; Shakila et al., 2009), such as trout fillets (Gonzalez-Fandos et al., 2004), salmon (Garcia-Linares et al., 2004, Gonzalez-Fandos et al., 2005; Diaz et al., 2009) and carp (Can, 2011) and there is no evidence of the application of this technology in molluscs. AIM Evaluate the effect of SVCC technology on the microbiological characteristics of mussels (M. galloprovincialis). Fig. 1 Experimental design and analysis carried out. A B C D E Fig. 2 Preparation of inoculated mussels (A), packaging (B), cooking (C), temperature monitoring (D), microbiological analysis (E). RESULTS TRIAL 1. EFFECT OF SOUS VIDE COOK AND CHILL ON INOCULATED MUSSELS Fig. 1 Effect of processing condition on microbiological population of inoculated mussel (IM) with Pseudomonas spp.. RM, raw mussel; IRM, inoculated raw mussel; IM75/10-30, inoculated mussel and processed at 75°C for 10 or 30 min. Data are mean values ±SD of 3 pouchs per group. Fig. 2 Effect of processing condition on microbiological population of inoculated mussel (IM) with Pseudomonas spp.. RM, raw mussel; IRM, inoculated raw mussel; IM85/10-30, inoculated mussel and processed at 85°C for 10 or 30 min. Data are mean values ±SD of 3 pouchs per group. Fig. 3 Effect of processing condition on microbiological population of inoculated mussel (IM) with Pseudomonas. RM, raw mussel; IRM, inoculated raw mussel; IM95/10-30, inoculated mussel and processed at 95°C for 10 or 30 min. Data are mean values ±SD of 3 pouchs per group. TRIAL 2. EFFECT OF SOUS VIDE COOK AND CHILL ON MUSSELS NATURAL BACTERIAL CONTAMINATION Fig. 4 Effect of processing condition on microbiological population of mussel natural bacterial load. RM, raw mussel; IRM, inoculated raw mussel; IM75/10-30, inoculated mussel and processed at 75°C for 10 or 30 min. Data are mean values ±SD of 3 pouchs per group. Fig. 5 Effect of processing condition on microbiological population of mussel natural bacterial load. RM, raw mussel; IRM, inoculated raw mussel; IM85/10-30, inoculated mussel and processed at 85°C for 10 or 30 min. Data are mean values ±SD of 3 pouchs per group. Fig. 6 Effect of processing condition on microbiological population of mussel natural bacterial load. RM, raw mussel; IRM, inoculated raw mussel; IM95/10-30, inoculated mussel and processed at 95°C for 10 or 30 min. Data are mean values ±SD of 3 pouchs per group. All t/T combinations resulted in a substantial reduction of the Pseudomonas spp. population (<1 log CFU/g) both in raw (4.9 log CFU/g) and experimentally inoculated (5.7 log CFU/g) mussels. The homogenate pH values (6.22) resulted not affected by the heat treatment (data not shown). Such results were confirmed on natural microbial contamination of mussel. A TBC reduction (2.4 log and 3.5 log, respectively) was registered after treatment at 85 °C both for 10 and 30 min, while the highest reduction (4.8 log) was observed at 95 °C for 10 min confirming the efficacy of sous vide cook treatments even for fresh mussels. This study was supported by Regione Autonoma Friuli Venezia Giulia (L.R.26/05) CONCLUSIONS REFERENCES The present study confirmed the effectiveness of SVCC technology for mussels to reduce the microbial population present in raw mussels. Technological and sensorial traits, as well the stability of these products both under refrigeration and thermal abuse conditions during storage need to be evaluated. Can Ö. P. (2011). World Academy of Science, Engineering and Tecnology, 56, 1225-1230; Diaz et al., 2009. Fishing Chimes, 23 (1), 131-136; Espinosa et al., 2016. Food Science and Technology International; Garcia-Linares et al., 2004. Journal of Food Quality, 27, 371–387; Gonzalez-Fandos et al., 2004. Food Microbiology, 21,193–201; Gonzalez-Fandos et al., 2005. Food Control 16, 77–85; Hansen et al., 1995. International Journal of Food Science and Technology, 30, 365–378; Rhodehamel, 1992. Food Technology, 46, 73–76; Shakila et al., 2009. International Journal of Food Science an Technology, 44, 2120-2126.