3. Bowel assays & crc risk:

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Presentation transcript:

3. Bowel assays & crc risk: 1. Sample collectioN: Normal colorectal mucosa from endoscopic procedures at the John Radcliffe hospital. - Patients given informed consent - COLlected 10ml blood and/or colorectal biopsies - Biopsies freshly used to extract DNA + RNA - remaining tissue snap frozen 2. ASSAY OPTIMIZATION: DNA - EXTRACTION AND QC - 23 Risk SNPS  polygenic risk score(PRs) - telomere length (tl) RNA - retrotranscription and DNAse treatment - pathway expression analysis (pea) BLOOD - NUCLEATED CELL SEPARATION - DNA EXTRACTION AND QC - RNA EXTRACTION AND QC colorectal biopsies - tissue fragmentation & homogenisation   3. Bowel assays & crc risk: compared levels of 3 assays in a group of healthy controls vs. patients with polyps/CRC - 2-stage design: pilot50 (50 cases & 50 controls) phase2 (182 cases & 57 controls) - cases showed longer tl than controls (OR=1.106; p=0.025) - cases presented with higher number of risk alleles (p=0.007; OR=1.086) 4. Blood assays: analyze correlation between bowel markers and their blood counterparts - blood tl was significantly correlated with bowel tl - BLOOD TL could be used as a proxy for risk assessment in a quick and less invasive way