National Institute of Legal Medicine and Forensic Sciences Lisbon Project HOME/2014/JDRU/AG/DRUG/7086 Identification and assessment of new psychoactive substances: a new European network National Institute of Legal Medicine and Forensic Sciences Lisbon Portugal Mário Dias Workshop 1 – Milan 29 Feb. – 1 Mar. 2016

Project HOME/2014/JDRU/AG/DRUG/7086 Identification and assessment of new psychoactive substances: a new European network 1. NILMFC The National Institute of Legal Medicine and Forensic Sciences (NILMFC) is a public institute, endowed with corporate entity and administrative and financial autonomy, subject to the supervision and guardianship of the Minister of Justice. It is an institution that is responsible for the co-ordination, guidance and supervision of all the country activities related with forensic expertises and other forensic activities. Workshop 1 – Milan 29 Feb. – 1 Mar. 2016

Drugs of Abuse; Medicines; Pesticides; Volatiles Project HOME/2014/JDRU/AG/DRUG/7086 Identification and assessment of new psychoactive substances: a new European network Post mortem Toxicology Clinical Toxicology (Emergence; Therapeutic Drug Monitoring) Human Performance Toxicology (Workplace Drug Testing; Driving Drugs of Abuse; Medicines; Pesticides; Volatiles 2. DCFT ≈ 65.000 analysis / year GC-MS (8) GC-MS/MS (1) UPLC- MS/MS (3) LC-MS/MS (1) Workshop 1 – Milan 29 Feb. – 1 Mar. 2016

Project HOME/2014/JDRU/AG/DRUG/7086 Identification and assessment of new psychoactive substances: a new European network 3. Key Persons Mário Dias Forensic Toxicologist in the NILMFC since 1983 Graduation in Pharmacy and Master in Legal Medicine and Forensic Sciences Director of the Chemistry and Forensic Toxicology Department of NILMFC (2000-2014) Senior Adviser of the Chemistry and Forensic Toxicology Department of NILMFC Member of the United Nations Office on Drugs and Crime (UNODC) International Informal Scientific Network Expert of the Reitox National Focal Point for the key indicator on drug-related deaths and member of the National Network for the Early Warning System on the NPS Coordinator in Portugal of the European project DRUID (Driving Under the Influence of Drugs) Invited Professor of Forensic Toxicology of Instituto de Ciências da Saúde Egas Moniz Workshop 1 – Milan 29 Feb. – 1 Mar. 2016

Project HOME/2014/JDRU/AG/DRUG/7086 Identification and assessment of new psychoactive substances: a new European network 3. Key Persons João Franco Forensic Toxicologist in the NILMFC since 1992 Graduation in Pharmacy and Master in Legal Medicine and Forensic Sciences Director of the Chemistry and Forensic Toxicology Department of NILMFC since 2014 Assessor in the Portuguese Accreditation Body since 2002 Technical assessor in the World Anti-Doping Agency since 2005 Susana Simões Forensic Toxicologist in the NILMFC since 2005 Graduation in Chemistry and Master in Legal Medicine and Forensic Sciences Expert Analyst in LC-MS/MS in the Lisbon Laboratory of Anti-Doping (1999-2005) Expert Analyst in LC-MS/MS in the DCFT of the NILMFC since 2005 Responsible for development of methods in LC-MS/MS in the projects DRUID and SCORE-COST-ES1307 Workshop 1 – Milan 29 Feb. – 1 Mar. 2016

Project HOME/2014/JDRU/AG/DRUG/7086 Identification and assessment of new psychoactive substances: a new European network 3. Activities Postmortem Toxicology: Expert activity on toxicology analysis in postmortem samples (search of drugs of abuse, including some NPS, medicines, pesticides and volatiles). Clinical Toxicology: Analysis in emergency service context, programs of monitoring therapeutic drugs in patients in cancer treatments and in victims of sexual assault. Human Performance Toxicology: Analysis in the context of Workplace Drug Testing Programs and Driving Under the Influence of Psychoactive Substances. Wastewater analysis in the context of the project ANALYSIS OF ILLICIT DRUGS IN EUROPEAN CITIES FROM WASTEWATERS - 2014 (SCORE-COST-ES1307). Workshop 1 – Milan 29 Feb. – 1 Mar. 2016

Project HOME/2014/JDRU/AG/DRUG/7086 Identification and assessment of new psychoactive substances: a new European network 3. Activities Validation and application of an UPLCMS/MS method for the quantification of synthetic cannabinoids in urine samples and analysis of seized materials from the Portuguese market. Susana Sadler Simões, Inês Silva, António Castañera, Mário Dias. Forensic Science International, 2014, 243: 117-125. Prevalence of alcohol, illicit drugs and psychoactive medicines in killed drivers in four European countries. Sara-Ann Legrand, Hallvard Gjerde, Cristina Isalberti, Trudy Van der Linden, Pirjo Lillsunde, Mario J. Dias, Susanne Gustafsson, Gunnel Ceder and Alain G. Verstraete. International Journal of Injury Control and Safety Promotion, 2014, 9;21(1):17-28. Analysis of amphetamine-type stimulants in biological matrices. Alexandra Gonçalves, Mário Barroso, Susana Simões, António Castañera, Mário Dias, Suzel Costa, José Nogueira. International Journal of Legal Medicine, 2012, 126;1, S268 Workshop 1 – Milan 29 Feb. – 1 Mar. 2016

Project HOME/2014/JDRU/AG/DRUG/7086 Identification and assessment of new psychoactive substances: a new European network 4. Foreseen activities in the framework of this project WorkStream 1 - Activity 2a (month 3 – month 14) Provide urine samples for qualitative analysis by HRMS key questions: Criterion for selection of samples; If necessary perform qualitative analysis of NPS by UPLC-MS/MS on forensic urine samples and urine samples collected in urgency room hospitals for common known NPS. key questions: List of standard substances available WorkStream 3 - Activity 2 (month 7 – month 18) Develop analytical method to perform qualitative analysis of NPS by UPLC-MS/MS on wastewater samples and urine samples collected in urgency room hospitals according to the list of core substances defined in WS1. Workshop 1 – Milan 29 Feb. – 1 Mar. 2016