PRACTICAL ON BLOOD PARASITES

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Presentation transcript:

PRACTICAL ON BLOOD PARASITES

Common methods for parasitological diagnosis of malaria The two methods common in use : 1: Light microscopy 2: Rapid diagnostic tests (RDTs).

Laboratory diagnosis of malaria Rapid diagnostic tests detect malaria antigens

RDT procedure

Laboratory diagnosis of malaria Light microscopy:1: Preparing blood film

Laboratory diagnosis of malaria Light microscopy:1: Preparing blood film

Laboratory diagnosis of malaria Light microscopy: Thick and thin films

Plasmodium falciparum (trophozoite stage in thick smear) Laboratory diagnosis of malaria Plasmodium falciparum (trophozoite stage in thin smear) Plasmodium falciparum (trophozoite stage in thick smear) CCMOVBD

The Malaria Parasite Three developmental stages seen in blood films: 10 Morphology of Malaria The Malaria Parasite CCMOVBD Trophozoites Three developmental stages seen in blood films: Trophozoite Schizont CCMOVBD CCMOVBD Gametocyte 2. Schizont 3. Gametocyte

11 Morphology of Malaria CCMOVBD Plasmodium vivax Species of malaria is identified by its characteristic microscopic appearance: CCMOVBD Plasmodium malariae Malaria Tutorials, Wellcome Trust Plasmodium ovale

Plasmodium falciparum (trophozoite stage in thin smear) 12 Morphology of Malaria Plasmodium falciparum (trophozoite stage in thin smear) CCMOVBD

Plasmodium falciparum (trophozoite stage in thick smear) 13 Morphology of Malaria Plasmodium falciparum (trophozoite stage in thick smear) CCMOVBD

Plasmodium falciparum 14 Morphology of Malaria Plasmodium falciparum (characteristic banana-shaped or crescent –shaped gametocyte stage in thin smear) CCMOVBD

A 25 year-old male from India, who came 3 months ago was admitted in KKUH with a history of severe anaemia and intermittent high grade fever for the last two months not responding to antibiotics. WHAT IS THE DIAGNOSIS? Diagnosis: malaria or Plasmodium vivax

A businessman who makes frequent trips to Thailand , presents with intermittent fever . WHAT IS THE DIAGNOSIS? Diagnosis: malaria or Plasmodium vivax

A student in KSU who returned three weeks from vacation in Africa , he developed intermittent fever last week and lost consciousness a short time ago. WHAT IS THE DIAGNOSIS? Diagnosis: malaria or Plasmodium falciparum

ARE THERE ANY PARASITES? WHAT STAGE ? The patient was then treated with schizontocidal antimalarial drugs, a follow-up blood film is shown . ARE THERE ANY PARASITES? WHAT STAGE ? Plasmodium flaciparum , gametocyte stage

Leishmania , amastigote stage A 7 year old child presented with anemia , hepatospenomegaly and fever .Not responding to antimalarials and antibiotics . Bone marrow smear is shown ARE THERE ANY PARASITES? WHAT STAGE ? Leishmania , amastigote stage

Bone marrow aspiration Bone marrow amastigotes