Figure 5. Effect of sugars on cAspAT mRNA in 3T3-F442A adipocytes

Slides:

Advertisements

Similar presentations

Figure 5. 1,25-(OH)2D plus Dex dose response for increased mMCSF by RT-PCR. Semiquantitative RT-PCR for mMCSF and GAP was performed in controls.

Advertisements

Figure 1. Schematic location of the amino acid substitutions and truncations of the androgen receptor. NTD, N-terminal domain; DBD, DNA binding domain;

Figure 2. Time-dependent effect of IL-6 on IGFBP-4 mRNA and protein in primary cultured hepatocytes. After 48 h of culture, hepatocytes were incubated.

Figure 1. Effect of the glucocorticoid (GC) cortisol at 1μ m on mac25/IGFBP-rP1 mRNA expression in cultures of Ob cells treated for 2, 6, 24, or 48 h.

Invest. Ophthalmol. Vis. Sci ;45(6): doi: /iovs Figure Legend:

From: MicroRNA let-7 Regulates 3T3-L1 Adipogenesis

Figure 4. Quantification of POMC mRNA in each cultured frog melanotrope cell subpopulation. The figure shows the POMC mRNA content in HD (solid bars) and.

Figure 1. DNA damage following treatment of human islets with and without a combination of three cytokines (IL-1β, 50 U/ml + TNF-α, 1000 U/ml + IFN-γ,

Fig. 1 HPLC and TLC analysis of[ 3H]25OHD3 metabolism in HKC-8 cells. A, Straight phase HPLC separation of radiolabeled vitamin D.

Figure 7. Cycloheximide blocks the potentiation of insulin action by MCC-555. Cardiomyocytes were subjected to the indicated incubation conditions using.

Volume 59, Issue 4, Pages (April 2001)

Figure 6. Assessment of endosomal acidic insulinase activity after a gel-filtration HPLC protocol and immunodepletion procedures. Endosomal acidic insulinase.

Fig. 4. PAR formation is dependent on topoisomerase II activity and required for PARP1 chromatin recruitment. A, 3T3-L1 preadipocytes were differentiated.

The Suppressor of Cytokine Signaling-3 Is Upregulated in Impaired Skin Repair: Implications for Keratinocyte Proliferation Itamar Goren, Andreas Linke,

Volume 133, Issue 2, Pages (August 2007)

Volume 140, Issue 2, Pages (February 2011)

Abdi Ghaffari, Ruhangiz T. Kilani, Aziz Ghahary

Matrix Metalloproteinase 9 Expression is Coordinately Modulated by the KRE-M9 and 12-O-Tetradecanoyl-Phorbol-13-Acetate Responsive Elements Takashi Kobayashi,

HIV-1 protease inhibitors decrease proliferation and induce differentiation of human myelocytic leukemia cells by Takayuki Ikezoe, Eric S. Daar, Jun-ichi.

Boye L. Jensen, Bianca Mann, Ole Skøtt, Armin Kurtz

BM-40(Osteonectin, SPARC) Is Expressed Both in the Epidermal and in the Dermal Compartment of Adult Human Skin Nicholas Hunzelmann, Martin Hafner, Sabine.

Volume 129, Issue 1, Pages (July 2005)

Interaction of HSV-1 Infected Peripheral Blood Mononuclear Cells with Cultured Dermal Microvascular Endothelial Cells: a Potential Model for the Pathogenesis.

Differential Expression of a Novel Gene in Response to hsp27 and Cell Differentiation in Human Keratinocytes Mojgan Hell-Pourmojib, Peter Neuner, Robert.

M.H.A. Baccora, P. Cortes, C. Hassett, D.W. Taube, J. Yee

Volume 44, Issue 3, Pages (November 2011)

Volume 53, Issue 5, Pages (May 1998)

Fibrin and Collagen Differentially Regulate Human Dermal Microvascular Endothelial Cell Integrins: Stabilization of αv/β3 mRNA by Fibrin1 Xiaodong Feng,

Xu Shi-wen, Christopher P. Denton, Alan M. Holmes, Carol M

In situ localization and quantification of seventy-two – kilodalton type IV collagenase in aneurysmal, occlusive, and normal aorta William D. McMillan,

Volume 133, Issue 6, Pages (December 2007)

Keratinocyte-Releasable Stratifin Functions as a Potent Collagenase-Stimulating Factor in Fibroblasts Aziz Ghahary, Feridoun Karimi-Busheri, Yvonne Marcoux,

Decreased Growth Inhibitory Responses of Squamous Carcinoma Cells to Interferon-γ Involve Failure to Recruit cki Proteins into cdk2 Complexes Beth L.

Shiga toxin 1 elicits diverse biologic responses in mesangial cells

Suppression of Vitamin D Receptor and Induction of Retinoid X Receptor α Expression During Squamous Differentiation of Cultured Keratinocytes Siegfried.

Expression of Cholesterol Sulfotransferase (SULT2B1b) in Human Skin and Primary Cultures of Human Epidermal Keratinocytes Yuko Higashi, Hirotoshi Fuda,

Volume 54, Issue 6, Pages (January 1998)

Epidermal growth factor and transforming growth factor α down-regulate human gastric lipase gene expression Eric Tremblay, Jean René Basque, Nathalie.

Volume 54, Issue 4, Pages (October 1998)

Stratifin-Induced Matrix Metalloproteinase-1 in Fibroblast Is Mediated by c-fos and p38 Mitogen-Activated Protein Kinase Activation Eugene Lam, Runhangiz.

Noritaka Oyama, Keiji Iwatsuki, Yoshimi Homma, Fumio Kaneko

Volume 59, Issue 2, Pages (February 2001)

Volume 57, Issue 3, Pages (March 2000)

Volume 61, Issue 1, Pages S106-S109 (January 2002)

Amplification and Overexpression of the EMS 1 Oncogene, a Possible Prognostic Marker, in Human Hepatocellular Carcinoma Bao-Zhu Yuan, Xiaoling Zhou,

Volume 56, Issue 2, Pages (August 1999)

Human Keratinocytes Respond to Osmotic Stress by p38 Map Kinase Regulated Induction of HSP70 and HSP27 M. Garmyn, A. Pupe Journal of Investigative Dermatology

Nicotine and cotinine stimulate secretion of basic fibroblast growth factor and affect expression of matrix metalloproteinases in cultured human smooth.

Supplementary Figure 1 A B C SW620 HT29 SW620

Ken Inoki, Masakazu Haneda, Shiro Maeda, Daisuke Koya, Ryuichi Kikkawa

Post-Transcriptional Regulation of UV Induced TNF-α Expression

Volume 70, Issue 5, Pages (September 2006)

Sequential effects of high glucose on mesangial cell transforming growth factor-β1 and fibronectin synthesis Jong Hoon Oh, Hunjoo Ha, Mi Ra Yu, Hi Bahl.

Volume 53, Issue 6, Pages (June 1998)

Volume 55, Issue 2, Pages (February 1999)

Volume 55, Issue 4, Pages (April 1999)

J. Martin, T. Bowen, R. Steadman Kidney International

Caveolin-1 downregulated survivin expression in cells with enhanced or constitutive activity via the Wnt pathway. Caveolin-1 downregulated survivin expression.

Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased survivin expression. Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased.

CPPED1 (A) and PPARγ2 (B) mRNA expressions in cultured SGBS cells during adipocyte differentiation. CPPED1 (A) and PPARγ2 (B) mRNA expressions in cultured.

Volume 73, Issue 6, Pages (March 2008)

Ctip2 directly regulates Notch1 expression in differentiated cells.

Oligomeric Aβ42 treatment induces expression of Ras and Cyclin D1 and activation of ERK in primary rat cortical neurons. Oligomeric Aβ42 treatment induces.

Fig. 1. Stomatin expression in 3T3-L1 adipocytes

TGF-β1 down-regulates induced expression of both class II MHC and B7-1 on primary murine renal tubular epithelial cells Nazifa Banu, Catherine M. Meyers

Northern blot analysis of maspin in cancer cell lines and human tissues. Northern blot analysis of maspin in cancer cell lines and human tissues. A, HMECs.

SIN4 function was required for the entry into a normal stationary phase. SIN4 function was required for the entry into a normal stationary phase. (A) The.

Suppression of Keratinocyte Growth and Differentiation by Transforming Growth Factor β1 Involves Multiple Signaling Pathways Alison L. Dahler, Lois L.

Fig. 1 Obesity-related adipocyte degeneration and cfDNA release.

Heparin-Binding EGF-Like Growth Factor Is Induced by Disruption of Lipid Rafts and Oxidative Stress in Keratinocytes and Participates in the Epidermal.

Presentation transcript:

Figure 5. Effect of sugars on cAspAT mRNA in 3T3-F442A adipocytes Figure 5. Effect of sugars on cAspAT mRNA in 3T3-F442A adipocytes. 3T3-F442A adipocytes were maintained for 24 h in culture medium in which glucose was either omitted (CTL) or changed to 25 mm of the indicated sugars. RNA was analyzed by Northern hybridization with a labeled cAspAT cDNA probe. Data obtained by scanning densitometry, was normalized for RNA loading using theβ -Actin mRNA signal. Data are expressed as the percent of cAspAT signal from 24-h glucose-deprived cells. Each value represents the mean ± sem of data obtained from at least three independent experiments with duplicate dishes. d-gluc, d-glucose; l-gluc, l-glucose; 3-OMG, 3-orthomethyl glucose; 2-DOG, 2-deoxyglucose. Identification of an Adipocyte-Specific Negative Glucose Response Region in the Cytosolic Aspartate Aminotransferase Gene**This work was supported by Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, and the Université Paris-Val de Marne. Endocrinology. 1998;139(12):4936-4944. doi:10.1210/endo.139.12.6342 Endocrinology | Copyright © 1998 by The Endocrine Society 1