PDCB BioC for HTS topic Understanding the tech. 01

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Next-Generation Sequencing: Methodology and Application

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Presentation transcript:

PDCB BioC for HTS topic Understanding the tech. 01 LCG Leonardo Collado Torres lcollado@wintergenomic.com lcollado@ibt.unam.mx August 25th, 2010

Topics Next generation sequencing technologies 454 SOLiD Pacific Biosciences Illumina Paired-end and Mate-pair Ion Torrent

NGS tech

NGS tech

454

SOLiD

Pacific Biosciences

Illumina

PE and MP

Ion Torrent

NGS tech

References 1. Mardis, E.R. Next-generation DNA sequencing methods. Annu Rev Genomics Hum Genet 9, 387-402 (2008). 2. Metzker, M.L. Sequencing technologies [mdash] the next generation. Nat Rev Genet 11, 31-46 (2010). 3. Tucker, T., Marra, M. & Friedman, J.M. Massively parallel sequencing: the next big thing in genetic medicine. Am. J. Hum. Genet 85, 142-154 (2009). 4. http://seqanswers.com/forums/index.php 5. http://www.iontorrent.com/ 6. http://seqanswers.com/forums/showthread.php?t=6447 7. http://seqanswers.com/forums/showthread.php?t=5250 8. http://pathogenomics.bham.ac.uk/hts/