A B PI: 2646 PI: 10 786 K-mer depth Figure S1. Estimation of Sogatella furcifera genome size based on flow cytometry and 17-mer statistics. (A) Estimation of Sogatella furcifera genome size based on flow cytometry. Comparing with the Drosophila melanogaster (D. mel) genome, the C value of the S. furcifera (S. fur) genome was estimated to be 0.75 pg. The genome size of S. furcifera was estimated to be 733Mb. (B) Estimation of S. furcifera genome size based on 17-kmer statistics. In total 56.76 Gb of short (<1kb) paired-end genome sequencing reads were used to generate the 17-mer sequences. The genome size of S. furcifera was estimated to be 730 Mb based on the formula: 17-mer number / 17-mer depth.

G protein-coupled receptor signaling pathway protein homo-oligomerzition adenylate cyclase-modulating G protein-coupled receptor signaling pathway Figure S2. Gene Ontology enrichment analysis for genes in expression pattern 1.

Figure S3. Gene Ontology enrichment analysis for genes in expression pattern 2.

oxidation–reduction process de novo IMP biosynthetic process Figure S4. Gene Ontology enrichment analysis for genes in expression pattern 3. The top 20 enriched terms are shown.

Figure S5. Gene Ontology enrichment analysis for genes in expression pattern 4.

Figure S6. Gene Ontology enrichment analysis for genes in expression pattern 5.

Figure S7. Gene Ontology enrichment analysis for genes in expression pattern 6.

oxidation–reduction process steroid hormone-mediated signaling pathway Figure S8. Gene Ontology enrichment analysis for genes in expression pattern 7.

cell–cell signaling oxidation–reduction process Figure S9. Gene Ontology enrichment analysis for genes in expression pattern 8. The top 20 enriched terms are shown.