Identification of Unknown Bacteria: individual assignment

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Presentation transcript:

Identification of Unknown Bacteria: individual assignment Lab 12 Goals and Objectives: Identification of Unknown Bacteria: individual assignment Use your data from the 12 possible unknowns for comparison and your Dichotomous Key to solve for your unknown based on the results you collect. From single colonies: Inoculate one MacConkey Agar (streak for isolation) Inoculate one Nutrient Gelatin Deep (stab) Make new streak for isolation on BHIA Make Gram Stain slides (at least 2 incase you drop one) record Gram reaction, size of cells, shape of cells, any arrangements you observe Test for catalase production on a slide

Unknown Identification Day 1: Each person sign out one unknown Make streak for isolation on BHIA Put your name on original slant and return Day 2: Gram stain Catalase test Inoculate MacConkey Agar Inoculate Gelatin Deep Day 3: Based on Gram, Gelatinase, Catalase, Lactose, set up assays to solve: A. If Staph: MSA and Coagulase B. If Strep/Entero: BAP and Bacitracin C. If Gram Neg: SIM, Citrate, MR, Urea

All 12 Possible Unknowns Gram Positive Gram Negative Gelatinase + Gelatinase - Gelatinase - Gelatinase + Catalase + Catalase - Lactose + Lactose - Mannitol + OR Coagulase + Mannitol - OR Coagulase -  Hemolytic OR Bacitracin R  Hemolytic OR Bacitracin S Indole + OR Citrate - Indole - OR Citrate + H2S + OR Motile H2S - OR Nonmotile Urea + OR MR + VP - Urea - OR MR - VP + Urea + Urea - Staphylococcus epidermidis Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pyogenes Klebsiella pneumoniae Salmonella typhimurium Enterobacter cloacae Bacillus subtilis Escherichia coli Proteus vulgaris Shigella flexneri

Identification of Unknown Bacteria: individual assignment Lab 12 Goals and Objectives: Identification of Unknown Bacteria: individual assignment Use your data from the 12 possible unknowns for comparison and your Dichotomous Key to solve for your unknown based on the results you collect. From single colonies: Inoculate one MacConkey Agar (streak for isolation) Inoculate one Nutrient Gelatin Deep (stab) Make new streak for isolation on BHIA Make Gram Stain slides (at least 2 incase you drop one) record Gram reaction, size of cells, shape of cells, any arrangements you observe Test for catalase production on a slide