How to perform an amniocentesis

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Presentation transcript:

How to perform an amniocentesis Mónica Cruz-Lemini, Miguel Parra-Saavedra, Virginia Borobio, Mar Bennasar, Anna Goncé, Josep M Martínez, Antoni Borrell.

How to: perform an amniocentesis Indications: ‪prenatal genetic studies, fetal infection, intraamniotic inflammation, fetal lung maturity Sign informed consent form: ideally 24hrs before the procedure Pre-procedure laboratories: RhD and viral (HBV, HCV and HIV) status Evaluate contraindications and drug adjustment When? The ideal gestational age is ≥ 16 weeks Machine settings: routine obstetric scan

Figure S1 Material requirements for performing an amniocentesis. Sterile gauze and antiseptic, sterile sheets, sterile ultrasound probe cover, sterile gel, 20 - 22G needle, two 10mL syringes or Vacutainer® 10mL tubes without additives (optional) and Luer adaptor attached to Vacutainer® holder (optional).

Figure S2 Transverse sweep of the maternal abdomen in a sagittal view. The ultrasound probe must be placed in a transverse plane to the maternal abdomen, always perpendicular to the maternal skin, taking care that it does not inadvertently slide.

Figure S3 Puncture of the maternal abdominal wall into the uterine cavity in a transverse view. Note the needle and the ultrasound probe at 45⁰ with regards to the mid-sagittal plane of the maternal abdomen.

Figure S4 Needle introduction into the uterine cavity and “tenting” image.

Figure S5 Amniotic fluid aspiration with the Vacutainer® system (optional).