From: Preservation of Retina Ganglion Cell Function by Morphine in a Chronic Ocular-Hypertensive Rat Model Invest. Ophthalmol. Vis. Sci.. 2012;53(7):4289-4298. doi:10.1167/iovs.12-9467 Figure Legend: Inhibition of TNF-α expression in the presence of morphine (1 mg/kg; IP) 3 days post injury. Rats were divided into two groups: ocular-hypertensive group (n = 4); and morphine-treated ocular-hypertensive group (n = 4). Three days post hypertonic-saline injection into the limbal veins, animals were euthanized and retina extracts (15 μg retina protein) were analyzed by Western blotting using selective anti-TNF-α primary antibodies. The signal was captured using appropriate secondary antibodies (HRP-conjugated; dilution 1:3000), enhanced chemiluminescent reagent, and the Biorad Versadoc imaging system. Data shown are representative of four, independent experiments. Data are expressed as mean ± SE. *P < 0.05; n = 4. Date of download: 10/21/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.