Institut Sains Biologi Muzium Zoologi Institut Sains Biologi Museum of Zoology
museumzoology.um.edu.my
Bulletin of the Museum of Zoology Next issue end of April 2013
Biodiversity seminars
Special exhibition
Workshop
Hunt for Vampire Moth
Biodiversity Genomics Lab With capacity for routine DNA barcoding of all animal groups
DNA barcoding of bats Funded by UMRG158/12SUS: Butterflies of Peninsula Malaysia Project
Testing the utility of DNA barcoding for rapid and accurate assessment of bat diversity in Malaysia in the absence of formally described species John James Wilson*, Sing Kong Wah*, Muhammad Rasul Abdullah Halim, Rosli bin Ramli, Rosli bin Hashim, & Mohd Sofian-Azirun Genetics and Molecular Research, in press
How to Rapidly Accelerate Biodiversity Inventory in Regions where Most of the Species are Unknown? Malaysia’s First DNA Barcoding Bioblitz John James Wilson, Kong Wah Sing, Khairunnisa Syaripuddin …..in preparation Funded by UMRG158/12SUS: Butterflies of Peninsula Malaysia Project
Time taken for completion of DNA barcoding Bioblitz Step Time taken (hrs) Specimen collection 12 Specimen sorting 2 DNA extraction 0.5 PCR set-up PCR 3 PCR check 1.5 2nd PCR set-up DNA sequencing 48 Sequence editing, alignment, upload 1 Analysis of DNA barcodes TOTAL 77.5 (3 days)
Cost of DNA barcoding Bioblitz Step Details of consumables/reagents Cost of consumables/reagents /service per specimen in MYR Specimen collection 1.5ml microcentrifuge tubes 0.07 Specimen sorting 96-well plate 0.14 DNA extraction Xytogen kits (100 preps), Pipette tips 2.33 PCR PCR Master mix, Primers, ddH20, 0.2ml PCR tube, Pipette tips 0.95 (0.95) PCR check Agarose, TAE buffer, DNA ladder (0.5μl), GelRed DNA stain 1.00 (1.00)* DNA sequencing 21.00 TOTAL 24.49 (26.49)
Building a DNA barcode reference library for the true butterflies (Lepidoptera) of peninsula Malaysia: What about the subspecies? John-James Wilson1,2, Kong-Wah Sing1, Mohd Sofian-Azirun2 PLoS ONE, in review Funded by UMRG158/12SUS: Butterflies of Peninsula Malaysia Project
Figure 5.