Patrick Van Der Voorn, Janneke Tekstra, Rob H. J. Beelen, Cornelis P

Slides:

Advertisements

Similar presentations

CC Chemokine Receptor 5 and CXC Chemokine Receptor 6 Expression by Lung CD8+ Cells Correlates with Chronic Obstructive Pulmonary Disease Severity Christine.

Advertisements

Date of download: 6/6/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Lessons Learned From Fatal Progressive Multifocal.

Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.

Heather Vinet-Oliphant, Xavier Alvarez, Elizabeth Buza, Juan T

Saskia Z. H. Rittersma, Martijn Meuwissen, Chris M

Lack of Complement Inhibitors in the Outer Intracranial Artery Aneurysm Wall Associates with Complement Terminal Pathway Activation Riikka Tulamo, Juhana.

Reduced Tumor Necrosis Factor-α and Transforming Growth Factor-β1 Expression in the Lungs of Inbred Mice that Fail to Develop Fibroproliferative Lesions.

Specific and Innervation-Regulated Expression of the Intermediate Filament Protein Nestin at Neuromuscular and Myotendinous Junctions in Skeletal Muscle

Papillary Carcinoma of the Thyroid

In Situ Expression of Interleukin-10 in Noninflamed Human Gut and in Inflammatory Bowel Disease Frank Autschbach, Jutta Braunstein, Burkhard Helmke,

Expression of the Hypoxia-Inducible and Tumor-Associated Carbonic Anhydrases in Ductal Carcinoma in Situ of the Breast Charles C. Wykoff, Nigel Beasley,

PTEN Mutational Spectra, Expression Levels, and Subcellular Localization in Microsatellite Stable and Unstable Colorectal Cancers Xiao-Ping Zhou, Anu.

Alvin Y. Liu, Martine P. Roudier, Lawrence D. True

VEGFR-3 and Its Ligand VEGF-C Are Associated with Angiogenesis in Breast Cancer Reija Valtola, Petri Salven, Päivi Heikkilä, Jussi Taipale, Heikki Joensuu,

CCL21 Expression Pattern of Human Secondary Lymphoid Organ Stroma Is Conserved in Inflammatory Lesions with Lymphoid Neogenesis Antonio Manzo, Serena.

Human Urinary Bladder Transitional Cell Carcinomas Acquire the Functional Fas Ligand during Tumor Progression Dominique Chopin, Reza Barei-Moniri, Pascale.

Stem Cell Marker Expression in Persistent Apical Periodontitis

Chemokine Receptor Expression on Neoplastic and Reactive T Cells in the Skin at Different Stages of Mycosis Fungoides Tilmann Kallinich, J. Marcus Muche,

Volume 108, Issue 1, Pages (July 1995)

Expression of PD-L1 in mastocytosis

TLR4, ATF-3 and IL8 inflammation mediator expression correlates with seizure frequency in human epileptic brain tissue Katharina Pernhorst, Stefan Herms,

Colocalization of Glial Fibrillary Acidic Protein, Metallothionein, and MHC II in Human, Rat, NOD/SCID, and Nude Mouse Skin Keratinocytes and Fibroblasts

Volume 125, Issue 1, Pages (July 2003)

Fibroblast Activation Protein: Differential Expression and Serine Protease Activity in Reactive Stromal Fibroblasts of Melanocytic Skin Tumors Margit.

Identification of the Tumor Cells in Peripheral T-Cell Lymphomas by Combined Polymerase Chain Reaction-Based T-Cell Receptor β Spectrotyping and Immunohistological.

Systemic Mastocytosis Associated with Chronic Idiopathic Myelofibrosis

Figure Neuroimaging and pathology

Expression and Role of IL-15 in Post-Burn Hypertrophic Scars

Placental pathology in egg donor pregnancies

Volume 71, Issue 3, Pages (February 2007)

Volume 76, Issue 5, Pages (September 2009)

Overexpression of Serpin Squamous Cell Carcinoma Antigens in Psoriatic Skin Atsushi Takeda, Dousei Higuchi, Tadahito Takahashi, Masashi Ogo, Peter Baciu,

Intraspinal AAV Injections Immediately Rostral to a Thoracic Spinal Cord Injury Site Efficiently Transduces Neurons in Spinal Cord and Brain Michelle.

Immunopathologic Features of Allergic Contact Dermatitis in Humans: Participation of Plasmacytoid Dendritic Cells in the Pathogenesis of the Disease?

Patrick R. Murray The Journal of Molecular Diagnostics

Jasper G. van den Boorn, Debby Konijnenberg, Trees A. M. Dellemijn, J

Association of intraluminal thrombus in abdominal aortic aneurysm with local hypoxia and wall weakening David A. Vorp, PhDa,b,c, Paul C. Lee, MDa, David.

Figure 1 Histopathologic features of a chronic active and a chronic plaque in the MS brain Histopathologic features of a chronic active and a chronic plaque.

Dennis R. Holmes, MD, William Wester, BA, Robert W

Volume 71, Issue 10, Pages (May 2007)

Fertility and Sterility

Figure Brain MRI and biopsy specimens from the pontine lesion

Molecular Aspects of Melanocytic Dysplastic Nevi

Volume 60, Issue 1, Pages (July 2001)

Massive Recruitment of Type I Interferon Producing Plasmacytoid Dendritic Cells in Varicella Skin Lesions Gianni Gerlini, Giulia Mariotti, Beatrice Bianchi,

Figure Nuclear Nrf2 expression after fumarate therapy A new left occipital fluid-attenuated inversion recovery hyperintense (A), T1 hypointense (B), and.

“Dermal Dendritic Cells” Comprise Two Distinct Populations: CD1+ Dendritic Cells and CD209+ Macrophages Maria Teresa Ochoa, Anya Loncaric, Stephan R.

Randall Adolph, BS, David A. Vorp, PhD, David L. Steed, MD, Marshall W

Challenges and Pitfalls in HNPCC Screening by Microsatellite Analysis and Immunohistochemistry Annegret Müller, Giuseppe Giuffre, Tina Bocker Edmonston,

Toshiyuki Yamamoto, Kiyoshi Nishioka

Figure 4 Neuropathology of MOG and AQP4 antibody–associated demyelinating lesions in the brain The biopsy specimen revealed a small actively demyelinating.

Silvina Gazzaniga, Alicia Bravo, Silvana R

Figure MRI and neuropathologic characteristics of the tumefactive demyelinating lesion in our patient MRI and neuropathologic characteristics of the tumefactive.

Role of CD8+ cells in the progression of murine adriamycin nephropathy

Long-term Improvements in Lifespan and Pathology in CNS and PNS After BMT Plus One Intravenous Injection of AAVrh10-GALC in Twitcher Mice Mohammad A.

Erratum The American Journal of Human Genetics

Successful Treatment of Alopecia Areata-Like Hair Loss with the Contact Sensitizer Squaric Acid Dibutylester (SADBE) in C3H/HeJ Mice P.I.A. Freyschmidt-Paul,

Volume 74, Issue 4, Pages (August 2008)

Role of Cytotoxic T Cells in Chronic Alopecia Areata

Volume 80, Issue 5, Pages (September 2011)

Alterations in Fibroblast α1β1 Integrin Collagen Receptor Expression in Keloids and Hypertrophic Scars Greg Szulgit Journal of Investigative Dermatology

Expression of the CD4+ Cell-Specific Chemoattractant Interleukin-16 in Mycosis Fungoides Volker Blaschke, Kristian Reich, Michael Letschert, Florian.

Volume 69, Issue 11, Pages (June 2006)

Figure 4 Autopsy immunochemistry results

An epithelial target site in experimental graft-versus-host disease and cytokine-mediated cytotoxicity is defined by cytokeratin 15 expression Diana.

Figure MRI brain comparison prior and after treatment and brain biopsy findings MRI brain comparison prior and after treatment and brain biopsy findings.

Lack of Membrane Expression of Interleukin-2 Receptor α Chain (CD25) in Mycosis Fungoides: Application of Laser Scanning Cytometry for Phenotyping of.

Quiz Page January 2010 American Journal of Kidney Diseases

Immunoglobulin-mediated CNS repair

Noncanonical Roles of Voltage-Gated Sodium Channels

Presentation transcript:

Expression of MCP-1 by Reactive Astrocytes in Demyelinating Multiple Sclerosis Lesions Patrick Van Der Voorn, Janneke Tekstra, Rob H.J. Beelen, Cornelis P. Tensen, Paul Van Der Valk, Corline J.A. De Groot The American Journal of Pathology Volume 154, Issue 1, Pages 45-51 (January 1999) DOI: 10.1016/S0002-9440(10)65249-2 Copyright © 1999 American Society for Investigative Pathology Terms and Conditions

Figure 1 Cryostat sections from active demyelinating MS lesions (case S59/S116) and chronic active demyelinating MS lesions (case S276/S283). A: Histochemical staining with the neutral lipid marker Oil Red O (ORO) in an active demyelinating lesion. Abundant lipid-filled ORO-positive perivascular and parenchymal macrophages are distributed throughout the demyelinated lesion (arrowheads). B: Immunohistochemical staining with the macrophage marker KP-1 (CD68) in an active demyelinating MS lesion. Strong immunoreactivity for KP1 was detected in both foamy perivascular and parenchymal macrophages (arrowheads). C: A higher magnification shows the CD68-immunoreactive macrophages (arrowheads). D: Immunohistochemical staining with KP1 MAb in a chronic active demyelinated MS lesion. Fewer KP-1-positive parenchymal macrophages were detected in the lesion center, whereas in the hypercellular rim abundant KP1 immunoreactivity was present in macrophages (arrowheads). LC, lesion center; HR, hypercellular rim. E: ORO staining of a chronic active demyelinating lesion. Degraded lipid was detected in the parenchymal foamy macrophages in the hypercellular rim (arrowheads). F: Immunohistochemical staining with a GFAP-specific PAb. Hypertrophic, reactive astrocytes were detected in the lesion center and the hypercellular rim surrounding the demyelinated region (arrowheads). Magnification, ×100 (B and D), ×200 (A, E, and F), and ×400 (C). The American Journal of Pathology 1999 154, 45-51DOI: (10.1016/S0002-9440(10)65249-2) Copyright © 1999 American Society for Investigative Pathology Terms and Conditions

Figure 2 Cryostat sections from a normal control case (S49) and an active demyelinating MS lesion (case S116). A: Immunohistochemical staining with a MCP-1 MAb on white matter brain tissue from a normal control case. No MCP-1-immunoreactive cells were found. B: Immunohistochemical staining with a MCP-1 MAb of an active demyelinating MS lesion. Strong to intense immunoreactivity for MCP-1 was detected in virtually all reactive astrocytes (arrowheads) in the surrounding tissue of a perivascular infiltrate located within the demyelinated region. PI, perivascular infiltrate. In perivascular and parenchymal macrophages (arrows) no immunoreactivity for MCP-1 was found. C: Immunohistochemical staining with the astrocytic marker GFAP. Immunoreactivity for GFAP was detected in reactive astrocytes (arrowheads) equally distributed throughout the lesion site. D: No specific immunoreactivity was found in an active demyelinating MS lesion using an isotype-specific control MAb (IgG1). Magnification, ×200. The American Journal of Pathology 1999 154, 45-51DOI: (10.1016/S0002-9440(10)65249-2) Copyright © 1999 American Society for Investigative Pathology Terms and Conditions

Figure 3 Cryostat sections from a chronic active demyelinating MS lesion (case S276). A: Immunohistochemical staining with a MCP-1-specific MAb. Reactive astrocytes (arrowheads) stain positive for MCP-1. The immunoreactivity for MCP-1 in the reactive astrocytes became stronger with distance from the lesion center. LC, lesion center; HR, hypercellular rim. B: A reactive astrocyte (arrowhead) at the inner edge of the hypercellular rim of the lesion is strongly immunoreactive for MCP-1. C: Immunohistochemical staining with a GFAP-specific PAb on serial sections. Hypertrophic, reactive astrocytes (arrowheads) were detected in the lesion center and the hypercellular rim surrounding the demyelinated region (arrowheads). D: In the normal-appearing white matter (NAWM) adjacent to the hypercellular rim no immunoreactivity for MCP-1 was detected. Magnification, ×100 (A and B) and ×200 (B and D). The American Journal of Pathology 1999 154, 45-51DOI: (10.1016/S0002-9440(10)65249-2) Copyright © 1999 American Society for Investigative Pathology Terms and Conditions