بسم الله الرحمن الرحيم

Dr.OSAMA AL-MUSHHADANY MRCS(ENGLAND) IRAQI BAORD PEDIATRIC SURGERY Nineva medical college

Sterilizations and disinfection Disinfection: is a process that kills or remove pathogenic M.O except spores. Disinfectant: A chemical agent used to achieve disinfection. The efficiency of disinfection depends on: 1- Nature and load of MO. 2- Duration of exposure to the chemical material 3- Temperature of the environment.

Methods of disinfection 1- Low temperature steam: dry steam at 73C° for 20 minutes at sub atmospheric pressure 2- Boiling water exposure to 100C° for 5 minutes at normal pressure. 3-Formaldehyde gas exposure in specialized cabinet at 50C°. 3-Glutaraldehyde 2% effective for heat sensitive material like flexible endoscopes, but its toxic and cause allergic reaction.

Sterilization: A process that destroy all form of microbial life including bacteria, virus, fungus and spores. Methods of sterilization: 1- autoclave: specialized container supply moist steam under pressure with high temperature. Used for metal ware, glassware, rubber and dry goods. The temp and time needed are: 3  minutes  at (132°C) 8  minutes  at (125°C), 15  minutes  at (118°C).   2-Ethylene oxide: A penetrative non corrosive gas used to sterilizes heat and moist sensitive substance. 3-Irradiation:Using gamma ray to sterilize syringes, catheters, cannulas and other plastic tubes and heat sensitive objects. 4- peracitec acid: chemical agent cause protein denaturation suitable for endoscopes.

Some chemical solutions used for skin preparation and wound cleaning 1- Povidone iodine: Can be used to disinfect skin before operation and to clean infected wounds and ulcers. Side effect: allergy, metabolic acidosis, interfere with thyroid function test, not recommended in pregnancy, not used inside abdominal cavities. 2- Chlorhixidine: Effective against gram positive MO. Can be used to disinfect skin before operation and to clean infected wounds und ulcers. 3- Alcohol: 70% used as disinfectant to prepare skin for operation, not used for wound or ulcer. 4- Hydrogen peroxide: Week disinfectant help to flush out depress from cavities by mechanical action

Prevention of wound infection at patient level 1- Avoid prolonged preoperative hospital stay. 2- Control any source of infection if possible. 3- Control DM, uremia, anemia, malnutrition, electrolyte disturbance. 4-Preoperative bathing. 5-Stop smocking 5- Avoid shaving the area of operation, if this is mandatory it should be on the table. 6- Prophylactic antibiotic. 7-Early post operative mobilization and exercise.

Prevention of wound infection at staff level 1-Staff with focus of infection should not deal with patients. 2-Decrease number of staff in the operative theatre with less movement as possible. 3-Warring of special clothes and gowns. 4- Using the head caps , face mask and boot. 5- Hand washing 6- Warring sterile gloves during surgical procedure and usual gloves when dealing with infective focus.

Prevention of wound infection at operative theatre level 1- The operative theatre design and building 2- The ventilation system 3- The table, light source, 4-Anesthetic machines and equipments, 5-Suckers and electro-cauterization devices. 6- Surgical instruments.

Prevention of wound infection at surgical word and medical devices level 1- Surgical word devices including: beds, mattresses, pillows, urinals, surgical truly, 2-Reception and examination rooms. 3- CanulLa, IV set line, drains, tubes, blood transfusion sets. 4- The visitors.

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