Lab 5: Photosynthesis.

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Presentation transcript:

Lab 5: Photosynthesis

Purpose To design and conduct an experiment to explore the effect of certain factors, including different environmental variables, on the rate of cellular photosynthesis To connect and apply concepts, including the relationship between cell structure and function (chloroplasts); strategies for capture, storage, and use of free energy; diffusion of gases across cell membranes; and the physical laws pertaining to the properties and behaviors of gases

Background Light Dependent Reactions: make O2 and ATP and NADPH Calvin Cycle: Makes G3P (Sugars) using CO2 How can we measure the rate of photosynthesis?

Procedure Hole punch leaves to make discs Use syringe to create a vacuum to suck the air out of the mesophyll in the leaves Put discs (will sink) into two different cups Control group: water + soap Experimental group: baking soda + soap Soap serves as a wetting agent coating the hydrophobic leaf surfaces and allowing the solutions to enter the cell Baking soda (sodium bicarbonate) serves a source of CO2 Put under light and watch the magic!

Measuring stuff! You will measure the amount of time it takes for the discs to start floating What makes them float? Design a graph to show time vs # floating discs

Design Your Own Experiment! The first procedure is for testing under normal circumstances Then you must design an experiment to test something else See me for a list of things to test! Lab write-up will be a Scientific Poster Present your designed experiment to the class