Usage Guidelines for Jeopardy PowerPoint Game Game Setup Right now, Click File > Save As, and save this template with a different file name. This will keep the template untouched, so you can use it next time! Scroll through the presentation and enter the answers (which are really the questions) and the questions (which are really the answers). Enter in the five category names on the main game board (Slide 4). Game Play Open 2nd Slide, let the sound play. Click to 3rd Slide, let the sound play. Click to 4th Slide and show students the Game Board As you play the game, click on the YELLOW DOLLAR AMOUNT that the contestant calls, not the surrounding box. When the student answers, click anywhere on the screen to see the correct answer. Keep track of which questions have already been picked by printing out the game board screen (Slide 4) and checking off as you go. Click on the “House / Home Icon” box to return to the main scoreboard. Final Jeopardy – Go to Slide 3 and click “Final Jeopardy” button in the bottom right corner, click again for the Question, click again for final jeopardy sound, When that is finished playing click again for the answer slide.

Enzymes Dude, So many Enzymes Rules, Directions Words & (Vocabulary) Images Potpourri 100 100 100 100 100 200 200 200 200 200 300 300 300 300 300 400 400 400 400 400 500 500 500 500 Final

Enzymes Dude, So many Enzymes Rules, Directions Words & (Vocabulary) Images Potpourri Final jeopardy 100 100 100 100 100 200 200 200 200 200 300 300 300 300 300 400 400 400 400 400 500 500 500 500 500

This is the enzyme that lays down nucleotides, the team captain?

What is DNA polymerase III?

This is another name for gyrase.

What is topoisomerase?

This is a 2-enzyme complex, and what it contains.

What is a primosome, helicase and primase?

This is what RNA polymerase does and another name for it.

What is lay down RNA primer for DNA to extend--primase?

These are the two functions of DNA polymerase I.

What is to remove RNA primers and fills in small areas during replication and repair?

This enzyme unwinds DNA and separates the strands by breaking H-bonds.

What is helicase?

This seals the gaps between the sugar phosphate backbone and forms the final phosphodiester bonds between Okazaki fragments.

What is ligase?

DAILY DOUBLE DAILY DOUBLE

These are five enzymes involved in DNA replication and their functions?

Helicase-"unzip" or unwind the DNA helix at the replication fork Primase-synthesises RNA primers to act as a template for future Okazaki fragments to build on to. DNA Polymerase III-synthesizing nucleotides onto the leading end in the classic 5' to 3' direction. DNA Polymerase I-of synthesizing nucleotides onto primers on the lagging strand, forming Okazaki fragments. Ligase-“glues” together Okazaki fragments, an area that DNA Pol I is unable to synthesize. Telomerase-Catalyzes the lengthening of telomeres; the enzyme includes a molecule of RNA that serves as a template for new telomere segments. Nuclease- in charge of excising, or cutting out, unwanted or defective segments of nucleotides in a DNA sequence. Topoisomerase-This enzyme introduces a single-strand nick in the DNA, enabling it to swivel and thereby relieve the accumulated winding strain generated during unwinding of the double helix.

This is another name for endonucleases and what they do.

What is restriction enzyme and cut DNA at recognition sites?

This is the 3 protein conglomerate involved in initiating replication, what it contains, and what each does.

What is a replisome— helicase - breaks the hydrogen bonds between the complementary DNA strands gyrase - a topoisomerase that cuts the DNA strands just ahead of the replication fork to relax supercoiling resulting from unwinding the helix primase - lays down RNA nucleotides on the DNA template to form an RNA primer ?

(Directionally speaking), this is what DNA replication is said to be.

What is bidirectional?

This is the direction that Okazaki fragments are added.

What is 5’ to 3’.

(Referring to the original strand), which carbon --of the complementary strand of the leading strand --becomes available?

What is 3’?

This is a description of how the leading and lagging strand are either continuous or discontinuous.

continuous replication-On the leading strand, polymerases can attach new nucleotides in the 5' to 3' direction with no problem, and start a continuously growing chain of new DNA Lagging strand: On this strand, the 5' end is exposed. Incoming nucleotides cannot attach to the 5' end. A different process--discontinuous replication must be employed to create the matching complement to the lagging strand.

This is a drawing of DNA replication, showing the leading, lagging, and original strands, as well as their directions. Additionally, this drawing shows 4 enzymes involved in replication.

http://usmle. biochemistryformedics http://usmle.biochemistryformedics.com/wp-content/uploads/DNA-replication-components.png

What this is.

What is a theta structure?

This is what the pink and green thing is.

What are primase and helicase?

These are DNA sequences that vary between individuals, which can be described with different phenotypes.

What are polymorphisms???

This is a regulatory region.

What are regions of DNA that allow genes to be linked to its environment?

These are the characteristics of repetitive DNA.

What are: 1. It accounts for more than 20% of our genome 2 What are: 1. It accounts for more than 20% of our genome 2.Its function is unknown 3.also known as selfish or parasitic DNA 4. is self-replicating 5. is associated with some heritable diseases such as Huntington’s disease.

The human genome has this many base pairs.

What is 2.9 billion?

This is what semi-conservative means.

What’s Resulting DNA after replication is half old, half new.?

This prevents rejoining of denatured/separated strands of DNA.

What’re helix destabilizing proteins?

This is how RNA is involved in replication.

What is: RNA serves as a primer to initiate DNA replication… What is: RNA serves as a primer to initiate DNA replication….built/laid down by primase

This is what we wouldn’t need/what would NOT exist if DNA could add nucleotides in both 5’ to 3’ and 3’ to 5’ directions.

What’re Okazaki Fragments

This is what Messelson and Stahl did and found out.

What is: 1. Synthesized DNA in two separate E What is: 1. Synthesized DNA in two separate E. coli cultures supplied with either heavy nitrogen (15N) or regular nitrogen (14N) for many generations 2. Isolated DNA from both cultures 3. Placed samples of DNA from both cultures in a centrifuge tube containing CsCl (same density as normal/ DNA) 4. When centrifuged, the DNA formed "light" and "heavy" bands, showing that the DNA could be separated with this method Their results showed that DNA replication was semi-conservative