W7 3/20 WHOT Copy down the following sequence of DNA and complete the complete the other half ATTCAGATC

W7 3/21 WHOT Please turn in your toothpickase Lab. List 3 things you learned from the video yesterday. Weren’t paying attention? Sad. Absent? The link is on the SWIFT, you need to watch the video and complete the WHOT before the end of the week.

DNA Structure Review 5’ These three components make up the nucleotide subunits of a DNA strand: The nucleotides on each strand are held together using this type of bond: If one strand is 5’ ATGTCA 3’, then the complimentary strand will be: The two complimentary strands are held together using this type of bond: Covalent Hydrogen 3’

“It has not escaped our notice” That the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.

Helicase Helicase breaks DNA into 2 separate strands. (unzips it) Hydrogen Bonds

DNA Polymerase DNA Polymerase adds on the complimentary bases to the new strands of DNA DNA Polymerase builds the other side of the DNA A T Covalent bond

DNA Structure Review This enzyme separates the two strands of DNA during DNA replication: During DNA replication, this enzyme adds nucleotides to a growing strand of DNA using the rules of complimentary base pairing: DNA replication always proceeds in this direction:

3/22 What are 2 enzymes involved in DNA replication: What are they, and what do they do?

DNA Replication, How? DNA is replicated Semi-conservatively.

DNA replication DNA Polymerase: New strands of DNA are synthesized from the 5’  3’ direction.

DNA replication: DNA Replication happens on both strands, in both directions: http://www.youtube.com/watch?v=hfZ8o9D1tus&feature=related But it’s not that simple: http://www.youtube.com/watch?v=4jtmOZaIvS0&feature=related Complimentary base-pairing (note that there is a mistake in this video!): http://www.youtube.com/watch?v=rpwjZX_z5rg Okazaki fragments http://www.youtube.com/watch?v=teV62zrm2P0

Replication in Action Replication occurs simultaneously at multiple places along a DNA strand. Replication is as fast as 50 base pairs a second! Humans have 3 billion Base pairs. Note the liNobel e-Museum: DNA nk

3/23 WHOT Describe what “semi-conservative” replication is.

Title: Linking Biology Ideas Together (Cell Division) Cell Size In the circle: Write everything you know about these topics. On the line: Write how the two topics in the circle are connected. How is new genetic material made and transferred to new cells? DNA Replication Enzymes

Instructions: Create a detailed paragraph answering our Essential Question. Include important concepts about each idea in the bubble and the connecting ideas. You may use the template below to help organize your thoughts.

DNA is Universal DNA structure - the double helix: same in all organisms DNA replication process: essentially the same in all organisms. All organisms need to unwind their DNA All organisms use one “old” strand as a template to make a “new” strand All organisms use the same nucleotides - ATGC (Though, as demonstrated by Chargoff, in different ratios) All DNA polymerases work in the 5’  3’ direction DNA polymerase from bacteria is so similar to human DNA polymerase that it can be used to copy the DNA of humans!

Cracking the Code

W7 3/20 WHOT Copy down the following sequence of DNA and complete the complete the other half ATTCAGATC

W7 3/21 WHOT Please turn in your toothpickase Lab. List 3 things you learned from the video yesterday. Weren’t paying attention? Sad. Absent? The link is on the SWIFT, you need to watch the video and complete the WHOT before the end of the week.

3/22 What are 2 enzymes involved in DNA replication: What are they, and what do they do?

3/23 WHOT Describe what “semi-conservative” replication is.