Volume 206, Issue 6, Pages 217-221 (June 2013) MicroRNAs as diagnostic markers for pancreatic ductal adenocarcinoma and its precursor, pancreatic intraepithelial neoplasm  Yue Xue, Ahmad N. Abou Tayoun, Kristine M. Abo, J. Marc Pipas, Stuart R. Gordon, Timothy B. Gardner, Richard J. Barth, Arief A. Suriawinata, Gregory J. Tsongalis  Cancer Genetics  Volume 206, Issue 6, Pages 217-221 (June 2013) DOI: 10.1016/j.cancergen.2013.05.020 Copyright © 2013 Elsevier Inc. Terms and Conditions

Figure 1 PDAC (H & E staining). (A) well-differentiated; (B) moderately differentiated; (C) poorly differentiated. Cancer Genetics 2013 206, 217-221DOI: (10.1016/j.cancergen.2013.05.020) Copyright © 2013 Elsevier Inc. Terms and Conditions

Figure 2 Expression of miR-148a level in neoplastic and non-neoplastic pancreatic parenchyma. (A) log2 (2−ΔCq) values for NP, CP, PanIN, and PDAC were calculated as shown in Materials and methods (*P < 0.05, **P < 0.0001, two-sample t-test for comparison with NP). Mean ± SEM is shown. (B) log2 (2−ΔCq) values for N and PDAC (**P < 0.0001, paired t-test for comparison with N). Mean ± SEM is shown. Abbreviations: NP, normal pancreatic parenchyma; CP, chronic pancreatitis; PanIN, pancreatic intraepithelial neoplasm; PDAC, pancreatic ductal adenocarcinoma; N, paired pancreatic tissue with negative margin. Cancer Genetics 2013 206, 217-221DOI: (10.1016/j.cancergen.2013.05.020) Copyright © 2013 Elsevier Inc. Terms and Conditions

Figure 3 Expression of miR-217 level in neoplastic and non-neoplastic pancreatic parenchyma. (A) log2 (2−ΔCq) values for NP, CP, PanIN, and PDAC were calculated as shown in Materials and methods (**P < 0.0001, two-sample t-test for comparison with NP). Mean ± SEM is shown. (B) log2 (2−ΔCq) values for N and PDAC (**P < 0.0001, paired t-test for comparison with N). Mean ± SEM is shown. Abbreviations: NP, normal pancreatic parenchyma; CP, chronic pancreatitis; PanIN, pancreatic intraepithelial neoplasm; PDAC, pancreatic ductal adenocarcinoma; N, paired pancreatic tissue with negative margin. Cancer Genetics 2013 206, 217-221DOI: (10.1016/j.cancergen.2013.05.020) Copyright © 2013 Elsevier Inc. Terms and Conditions

Figure 4 Expression of miR-196a, miR-21, and miR-10b in neoplastic and non-neoplastic pancreatic parenchyma. log2 (2−ΔCq) values for N, PanIN, and PDAC were calculated as shown in Materials and methods (*P < 0.05, **P < 0.0001, two-sample t-test for comparisons between PanIN and N, paired t-test for comparisons between PDAC and N). Mean ± SEM is shown. Abbreviations: NP, normal pancreatic parenchyma; CP, chronic pancreatitis; PanIN, pancreatic intraepithelial neoplasm; PDAC, pancreatic ductal adenocarcinoma; N, paired pancreatic tissue with negative margin. Cancer Genetics 2013 206, 217-221DOI: (10.1016/j.cancergen.2013.05.020) Copyright © 2013 Elsevier Inc. Terms and Conditions