EQUIPMENTS
Autoclave Incubator Centrifuge Balances pH Meter Micropipette Refrigerators & Freezers Cryo storage Containers Cell Counter Dissecting Microscope
AUTOCLAVE Pressure: 15 pound(lb)/inch2 in excess of atm Temp. : 121 C EXPOSURE for 15 min. sufficient (sterlized) PRINCIPLE: Steam under Pressure
INCUBATOR Hot Room for Cell Culture Large enough: (~50-200L) 1.5 to 6 ft3 III. Controlled temp.
Humid CO2 Incubator Controlled atmosphere Mixture :Humidity & elevated co2 in Atm Controlled atmosphere
Magnetic Stirrer Rapid action for dissolving Chemicals i.e; enzymatic tissues Diasaggregation Suspension cultures
Cell-sediment satisfactorily at (80-100g) CENTRIFUGE To inc. conc. of cells Wash off reagent Cell-sediment satisfactorily at (80-100g)
BALANCES An accurate measure of Weighing Items
pH Meter To Check pH of : Media Special reagents
Micropipette A Pipettor To pipette conc. (~10-200microL) Sterilzed tips are used
REFRIGERATORS / FREEZERS Used for Storage In Hospitals , Blood Banks. Hygienic cold rooms
CRYOSTORAGE CONTAINERS Storage at extreme low Temp. Stored in: 35L Freezer In canes & canisters In drawers & racks
CELL COUNTER To precise quantitative growth kinetics Automated cell counting Size analysis
DISSECTING MICROSCOPE Dissecting Tissue’s small pieces Counting & picking of monolayer colonies
Hot-Plate Magnetic Stirrer To accelerate dissolutions of some reagents