Prof.Dr. Raúl Carlos López Sánchez Ecophysiological characterization of Coccoloba uvifera L.: the case of a salt and drought stress tolerant plant species for sand dune reclamation in Cuba Prof.Dr. Raúl Carlos López Sánchez Local promoter

Prof.Dr. Raúl Carlos López Sánchez Overall Academic Objective (OAO): strengthen research and training on abiotic, especially salt and drought, stress of economically interesting species at Granma University (GU). Overall Developmental Objective (ODO): improve capacity building for research in salt and drought stress at GU. Specific Academic Objective (SAO): strengthen research and training on ecophysiological, nutritional and phyto-chemical characterization and fruit production under salt and drought stress of C. uvifera in saline coastal soils of Cuba. Specific Developmental Objective (SDO): develop the capacities for ecophysiologically trained researchers to reduce climate change vulnerability of the coastal areas of Cuba. Prof.Dr. Raúl Carlos López Sánchez Local promoter

Prof.Dr. Raúl Carlos López Sánchez Intermediate Results (IR) IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known. IR2. Nutritional and phytochemical compounds of C. uvifera are characterized and known. IR3. The visibility of the Abiotic Stress Group of the Biotechnology Study Centre, as an excellence center for ecophysiological salt and drought stress research in Cuba has increased Prof.Dr. Raúl Carlos López Sánchez Local promoter

Prof.Dr. Raúl Carlos López Sánchez IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known. 1.1. Preparation of experiments 1.1.2 Literature review about drought and salt stress in relation to the selected and similar species Prof.Dr. Raúl Carlos López Sánchez Local promoter

1.1. Preparation of experiments North South IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known. 1.1. Preparation of experiments 1.1.3 Definition of experimental design and practical requirements Playa Guardalavaca, Holguin province Latitude: 21°07′30″N Longitude: 75°49′44″O East Playa Las Coloradas, Granma province Latitude: 19° 56' 00" N Longitude: 77° 41' 00" W Playa Ancon, Sancti Spiritus province Latitude: 21° 44' 00" N Longitude: 80° 01' 00" W Middle Cayo Coco, Ciego de Avila province Latitude: 22º 32' 10.06" N Longitude: 78º 21' 19.44" W West Playa Varadero, Matanzas province Latitude: 23°10'28"N Longitude: 81°12'43"W Playa Rancho Luna, Cienfuegos province Latitude: 22°02′20″N Longitude: 80°25′38″W

Prof.Dr. Raúl Carlos López Sánchez IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known. 1.1. Preparation of experiments 1.1.3 Definition of experimental design and practical requirements Prof.Dr. Raúl Carlos López Sánchez Local promoter

1.1. Preparation of experiments IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known. 1.1. Preparation of experiments 1.1.5 Training of the personnel responsible for the manipulation of the equipment and the measurements

Prof.Dr. Raúl Carlos López Sánchez IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known. 1.2. Experiments in controlled conditions 1.2.1 Evaluation of the response to salt and drought stress of C. uvifera during germination Prof.Dr. Raúl Carlos López Sánchez Local promoter

Prof.Dr. Raúl Carlos López Sánchez Control 5 dS.m-1 IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known. 1.2. Experiments in controlled conditions 1.2.1 Evaluation of the response to salt and drought stress of C. uvifera during germination 15 dS.m-1 25 dS.m-1 Salt stress Prof.Dr. Raúl Carlos López Sánchez Local promoter

50 % field capacity 100 % field capacity IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known. 1.2. Experiments in controlled conditions 1.2.1 Evaluation of the response to salt and drought stress of C. uvifera during germination Drought stress 0% field capacity

IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known. 1.2. Experiments in controlled conditions 1.2.2 Evaluation of ecophysiological responses (gas exchange, photosynthesis, chlorophyll fluorescence, plant-water-relations, leaf area index) of C. uvifera to salt and drought stress at seedling and sapling stage. Control 15 dS.m-1 Salt stress

IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known. 1.2. Experiments in controlled conditions 1.2.2 Evaluation of ecophysiological responses (gas exchange, photosynthesis, chlorophyll fluorescence, plant-water-relations, leaf area index) of C. uvifera to salt and drought stress at seedling and sapling stage. Control 15 dS.m-1 Salt stress Control 15 dS.m-1

IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known. 1.2. Experiments in controlled conditions 1.2.2 Evaluation of ecophysiological responses (gas exchange, photosynthesis, chlorophyll fluorescence, plant-water-relations, leaf area index) of C. uvifera to salt and drought stress at seedling and sapling stage. Salt stress

1.3. Experiments in field conditions. North South 1.3. Experiments in field conditions. 1.3.2 Fruit collection of C. uvifera accessions in selected ecosystems. Playa Guardalavaca, Holguin province Latitude: 21°07′30″N Longitude: 75°49′44″O East Playa Las Coloradas, Granma province Latitude: 19° 56' 00" N Longitude: 77° 41' 00" W

IR2. Nutritional and phytochemical compounds of C IR2. Nutritional and phytochemical compounds of C. uvifera are characterized and known. 2.1 Intensive literature review on C. uvifera about the nutritional and medicinal compounds

2.2 Evaluation of nutritional quality of C. uvifera IR2. Nutritional and phytochemical compounds of C. uvifera are characterized and known. 2.2 Evaluation of nutritional quality of C. uvifera 2.3 Phytochemical screening and antibacterial activity evaluation of C. uvifera   Metabolites North of east region Leaves Bark of the shaft Fruits DCM EtOH EtOAc Alkaloids - + ++ Coumarins Fatty Acid Resins Triterpenes/Steroids Saponines* (ultrasonido) -* Saponines* (maceración) +* Free amino acid Cardiotonic Glycosides Reducers Carbohydrates Phenols/Tanines +++ Quinones Antocianidines Flavonoids Better compounds Mucilages

Prof.Dr. Raúl Carlos López Sánchez Local promoter

Prof.Dr. Raúl Carlos López Sánchez Local promoter

Prof.Dr. Raúl Carlos López Sánchez Local promoter