Replication, Transcription and Translation Biology Powerpoint #2 – Chapter 12 Mr. Velekei

Vocabulary DNA Polymerase DNA Replication Semi-Conservative

Warm Up: Base Pairing 1. A C C T G A 2. G C T A G A G

3. Describe what a double helix would look like untwisted: Ladder, with bases for steps

4. What is the DNA ‘backbone’ made of? Phosphorous and Sugar

DNA Replication Nucleotide Hydrogen bonds Sugar-phosphate backbone Key Adenine (A) Thymine (T) Cytosine (C) Guanine (G)

1. What is DNA replication? Making a copy of DNA DNA replication is when DNA polymerase (an enzyme) opens the DNA molecule and matches complimentary bases to each base.

Mitosis with Replication 2 2 2 Makes 2 Identical Cells

Mitosis without Replication 1 2 1 Makes 2 Different cells with half as much DNA

3. Why is DNA replication important for cells and multicellular organisms? Replication allows cells to divide Forming cells that are genetically identical to the original

DNA Replication Original strand New strand Growth Replication fork DNA polymerase New strand Original strand Nitrogenous bases

4. When does DNA Replication occur? After the cell grows big enough to divide, during interphase, the “S” phase

5. What is a chromosome? A chromosome is DNA that is twisted up very tightly around histones

6. What is DNA Polymerase? Principal enzyme in DNA replication; adds nucleotides to growing strand of DNA and proofreads new strand of DNA

7. Draw a picture of a Chromosome before and after DNA replication

8. Drawings of the 3 steps of DNA Replication DNA Polymerase

9. Explain why DNA replication is considered ‘semi-conservative? In the new DNA created, one strand is from the original, and one is a new one. Semi = part of Conserve = save

Biology Powerpoint #2 – Chapter 12 Mr. Velekei Transcription Biology Powerpoint #2 – Chapter 12 Mr. Velekei

Vocabulary Exon Intron RNA Polymerase Transcription Uracil

RNA Where is DNA stored? ______________________ Nucleus What organelle makes proteins? ______________________ Where are proteins made? ______________________ Nucleus Ribosomes Cytoplasm

What problem might this pose? DNA can not take directions for making proteins to the ribosomes.

How does RNA solve this problem? RNA is a disposable copy of DNA that can leave the nucleus

RNA has ribose sugar instead of deoxyribose B. RNA’s structure is very similar to the structure of DNA except for 3 major differences: RNA has ribose sugar instead of deoxyribose

2. RNA is single-stranded 3. RNA has uracil (U) instead of thymine (T) DNA: AGTCCTTTAGT RNA: AGUCCUUUAGU

There are three main types of RNA:

1. Ribosomal RNA (rRNA)

2. Transfer RNA (tRNA)

3. Messenger RNA (mRNA)

III. Transcription Transcription: Producing RNA by copying part of the DNA’s nucleotide sequence RNA DNA RNA polymerase Adenine (DNA and RNA) Cystosine (DNA and RNA) Guanine(DNA and RNA) Thymine (DNA only) Uracil (RNA only)

Describe the process of transcription using drawings. RNA Polymerase rips open the DNA double helix RNA polymerase grabs bases and lines them up with the original DNA strand Half of the DNA is copied into a strand of mRNA, then the DNA strand closes, hydrogen bonds reform

How does RNA polymerase know where to start? Starts when it finds a “promoter” (specific base sequence) Found near the beginning of a gene sequence

Describe the process of RNA editing RNA editing is a process that occurs in the nucleus. It removes introns “intervening sequences” and leaves mRNA with only the exons “expressed sequences.” After editing a cap and tail are attached and the mRNA is ready to enter into the cytoplasm.

Biology Powerpoint #2 – Chapter 12 Mr. Velekei Translation Biology Powerpoint #2 – Chapter 12 Mr. Velekei

Vocabulary Anticodon ‘AUG’ Codon Polypeptide ‘Stop’ Codon Translation

IV. Translation Proteins are long chains of amino acids. Codon: 3 consecutive nucleotides that “code” for a specific amino acid. What is the universal “start” codon: AUG What are the three “stop” codons? UGA, UAA, UAG

The Genetic Code

The Genetic Code

Use the genetic code below to translate the following mRNA sequences: A U G U A A C G G G C A U U U U A A U C C A U G G A A G U G A U U C C A U A A C C A U G U G U C C C C A A U G A A A A

Use the genetic code below to translate the following mRNA sequences: A U G U A U C G G G C A U U U U A A U C C A U G G A A G U G A U U C C A U A A C C A U G U G U C C C C A A U G A A A A Methionine (START), Tyrosine, Arginine, Alanine, Phenylalanine, STOP. Serine, Methionine, Glutamic Acid, Valine, Isoleucine, Proline, STOP Methionine, Cysteine, Proline, Glutamine, STOP, Lysine

Translation: The decoding of RNA into a polypeptide chain (protein)

The Central Dogma of Biology is: DNA  RNA  protein Where does the first step take place? Nucleus Where does the second step take place? Cytoplasm

What is the job of tRNA during translation What is the job of tRNA during translation? Bringing amino acids to the ribosomes and match them up with the correct base on mRNA. What is an anticodon? The three bases on a tRNA that match with the mRNA codons. What is the role of the ribosome during translation? It is the site of protein assembly

H. 1) mRNA is transcribed in the nucleus then travels to the cytoplasm

2) Ribosome grabs mRNA. tRNA brings amino acids to the ribosome

3) tRNA matches with complimentary mRNA 3) tRNA matches with complimentary mRNA. Ribosome makes peptide bond between amino acids, and breaks the bond between tRNA and amino acid.

4) Peptide chain continues to grow until ribosome reaches a stop codon Protein is complete.