Types of Microscopes Unit 4 Biology

Simple Microscopes Have only 1 lens Give two examples of a simple microscope: Magnifying Glass Reading Glasses

Compound Microscopes Have more than one ______ lens

Dissecting Microscopes 20x 30x Magnify by 10x or ______ or ________ Shine light on or ____________ the specimen Specimen can be ________ or thin. through thick

Dissecting Microscope

Light Microscopes Use a beam of Light The specimen can be dead or alive

40- 400x through Light Microscope thin Magnify by ______________ Shines light ______________ the specimen Specimen must be _________. through thin

Light Microscope Image

electrons dead air water Electron Microscope Use a beam of _______________- The specimen must be _________ because an electron microscope is used in a vacuum with no _____ and no ________ dead air water

60,000x Scanning E.M. Makes 3D pictures of surfaces Magnify by _________ 60,000x

Scanning E.M. Image (3D)

Makes _____ pictures of thin slices Transmission E.M. 2D Makes _____ pictures of thin slices Magnify by __________ 100,000x

Transmission E.M. Image (2D) Butterfly Antenna

_____________ can be seen with these 2 types of microscopes. Atoms Other Microscopes Probe Microscopes Ion Microscopes _____________ can be seen with these 2 types of microscopes. Atoms

Probe Microscope

Probe Microscope Image

Ion Microscopes

Ion Microscope Images

Microscope Parts and Functions

1. Eyepiece Magnifies by 10x

Changes Objective Lens 2. Revolving Nose Piece Changes Objective Lens

3. Low power Objective Lens Magnifies by 4x (Red)

4. Medium Power Objective Lens Magnifies by 10x (yellow)

5. High Power Objective Lens Magnifies by 40x (Blue)

6. Stage Clips To hold slide in place

Adjusts the amount of light 7. Diaphragm Adjusts the amount of light

8. Light Light to see specimen

9. On/Off Switch Turns light on and off

10. Arm To hold while carrying

11. Stage To set slide on

To focus only when in Low Power 12. Course Adjustment To focus only when in Low Power

To focus when using medium or high power 13. Fine Adjustment To focus when using medium or high power

14. Base To hold while carrying

Both Arm Base Eyepiece Away Teacher Lens Tissue A. Carry a microscope with ___________ hands – one hand holding the _______ And the other hand under the ___________ Always hold the microscope upright so the _____________ cannot fall out When you place a microscope at your table, make sure it is _______ from the edge If there is anything wrong with the microscope, or it is damaged in any way, let the _______________ know immediately. B. To clean the objective lenses and eyepiece, use _______________________ Base Eyepiece Away Teacher Lens Tissue

Light Low 4X Slide Lens Highest Slide Coarse Focus Fine Diaphragm Prepare your slide Plug the microscope in. Turn on the ______________ Be sure the ___________ Power Objective Lens (_______) is clicked into place. NOTE: Be sure to look at the microscope from the side when changing objective lenses to avoid damaging the ________________ and _____________ 4. Use coarse focus to raise the stage to its ________________ position 5. Place the _______________ on the stage. Look through the eyepiece and turn the __________________ (lowering the stage) until the specimen is visible. 7. Use the ____________ adjustment to bring your specimen into sharp focus. You may wish to move the ________________ to adjust the amount of light. **The Eyepiece has a black arrow on it for pointing out parts of a specimen Light Low 4X Slide Lens Highest Slide Coarse Focus Fine Diaphragm

Focused Mid 10X Side Coarse Fine Focused Side High 40X Fine Low Cord 8. Make sure the specimen is ______________ 9. Switch to the ___________ Power Objective Lens ( ______) NOTE: Look at the microscope from the ____________ when you change lenses. 10. To avoid losing your focus and/or crushing the slide with the lens, never use the __________________ focus on medium or high power. Use the _____________ focus only to make the specimen visible. 11. Make sure the specimen is ______________. Look from the _________ as you change objective lenses and switch to the __________ Power Lens (________) 12. Use only the _____________ focus to make the specimen visible. 13. When finished, make sure the ___________ power objective lens is in place, wrap the __________ around the _________ of the microscope, cover, and place in the appropriate location Mid 10X Side Coarse Fine Focused Side High 40X Fine Low Cord Arm