Proper Use & Field of View

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Presentation transcript:

Proper Use & Field of View Using Your Microscope Proper Use & Field of View Geek Week - Microscope Randoms

Setting Up the Microscope Using the Microscope Setting Up the Microscope Use both hands to carry. One hand on the base, and the other on the arm. Place the microscope away from the edges of the desk If lenses are dirty, clean with lens paper only. Plug in microscope light so that the cord is not in your way Keep objects clear of desk and cords

Using the Microscope Low Power Focus Always start with the low-power objective lens (the shortest one) about 1cm above the stage. Carefully place the slide on the stage and centre it over the light source Use the stage clips to hold the slide in place While viewing, slowly adjust the focus with the coarse-adjustment knob. Use the fine-adjustment knob to sharpen the focus, and adjust the diaphragm to a suitable brightness.

Field of View Field of View (FOV) – The diameter of the circle of light that you see when looking into a microscope. Find a part of the sample that interests you, and on your handout, sketch what you see for the appropriate magnification (40x) Make sure the details in your drawing fill the same space in the circle as they do in the FOV Label your drawing

Field of View: Drawing Example Criteria Drawing is made with a sharp pencil. Size of the drawing is proportional to the way it appears under the microscope. Magnification and/or FOV diameter is printed below the circle. Title appears just below the magnification. A ruler is used to draw the lines for labels. Labels are printed neatly outside the FOV and parallel to the bottom of the page. No noticeable eraser marks. Measurements are printed within parenthesis to the right of the title.

Using the Microscope Medium Power Focus Once focused under low power, carefully turn the revolving nosepiece until the medium-power lens “clicks” Remember to not use the course-adjustment knob. Adjust the focus using only the fine-adjustment knob Complete your FOV sketch (100x magnification)

Using the Microscope High Power Focus Once focused under medium power, carefully turn the revolving nosepiece until the high-power lens “clicks” Remember to not use the course-adjustment knob. Adjust the focus using only the fine-adjustment knob Complete your FOV sketch (100x magnification)

Proper Care & Use of the Microscope Clean-Up Turn the revolving nosepiece to the low-power lens. Remove the slide from the stage. If the stage is wet, wipe it off with a paper towel. Wrap the electric cord around the microscope base and carry the microscope back to its proper place using both hands. Check this out: McDonald’s Chicken Nuggets Under the Microscope!