Albumin Analysis by Potentiometric Method

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Presentation transcript:

Albumin Analysis by Potentiometric Method

Properties of Albumin Half-life: 17 to 23 days Molecular weight: 68,000 Isoelectric point: 4.8

The Advantages of Potentiometric Method 1. Integrate easily 2. Microfabricate easily 3. Real time response

Materials Monomer Dimethylaminoethyl methacrylate-methyl chloride:DMAEMMC Dimethylaminopropyl methacrylamide:DMAPMA Crosslinking agent Tetraethylene glycol dimethacrylate:TEGDMA

Materials Initiators Cumene hydroperoxide:CHP Tetramethyl ethylenediamine:TMEDA Modification agent 2-mercapto ethanol 3-mercapto propionic acid n-dodecyl thiol

Materials Electrodes: Working : Au polymer film Counter: Pt Reference: Ag/AgCl Electrolyte: 0.5M KCl