Enzyme-linked immunosorbent assay (ELISA) for desmoglein 3

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Presentation transcript:

Enzyme-linked immunosorbent assay (ELISA) for desmoglein 3 Enzyme-linked immunosorbent assay (ELISA) for desmoglein 3. Anti-Dsg3 antibodies (αDsg3) from pemphigus serum binds Dsg3 on the ELISA plate; irrelevant antibodies, that do not bind, are washed off. The plate is then incubated with horseradish peroxidase (HRP) conjugated antihuman IgG, which binds the anti-Dsg3 IgG that is on the plate. HRP is an enzyme that turns a clear substrate blue and the amount of color, read on spectrophotometer, correlates with the amount of pemphigus (i.e., anti-Dsg3) antibody in the patient's serum. Source: Chapter 54. Pemphigus, Fitzpatrick's Dermatology in General Medicine, 8e Citation: Goldsmith LA, Katz SI, Gilchrest BA, Paller AS, Leffell DJ, Wolff K. Fitzpatrick's Dermatology in General Medicine, 8e; 2012 Available at: http://accessmedicine.mhmedical.com/DownloadImage.aspx?image=/data/books/gold8/gold8_c054f009.png&sec=41144133&BookID=392&ChapterSecID=41138753&imagename= Accessed: October 22, 2017 Copyright © 2017 McGraw-Hill Education. All rights reserved