Fig. 1. Development of an anti-FcεRI Fab fragment antibody NPB311

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Fig. 1. Development of an anti-FcεRI Fab fragment antibody NPB311 Fig. 1. Development of an anti-FcεRI Fab fragment antibody NPB311. (A) Human FcεRIα was coated on the plate, followed by incubation with the indicated antibodies. Specific antibody binding was detected by further incubation with hIgE, followed by incubation with horseradish peroxidase (HRP)-conjugated mouse-anti-hIgE and development with Immune Glo substrate. (B) hFcεRIα-expressing RBL-SX38 cells were seeded in a 96-well plate followed by incubation with the indicated antibodies. Specific binding was detected by further incubation with HRP-conjugated anti-hIgG and development by Immune Glo substrate. FcεRI, high-affinity IgE receptor I; hIgE, human immunoglobulin E; RBL, rat basophilic leukemia. Fig. 1. Development of an anti-FcεRI Fab fragment antibody NPB311. (A) Human FcεRIα was coated on the plate, followed by incubation with the indicated antibodies. Specific antibody binding was detected by further incubation with hIgE, followed by incubation with horseradish peroxidase (HRP)-conjugated mouse-anti-hIgE and development with Immune Glo substrate. (B) . . . Yonsei Med J. 2016 Nov;57(6):1412-1419. http://dx.doi.org/10.3349/ymj.2016.57.6.1412