Fig. 5. Evaluation of glycoprotein (GP)-specific IgG subclass (A) and its lowest concentration for GP binding (B). (A) Purified IgG (2 µg/mL) from clone A6-9 was reacted with GP1e, followed by incubation with horseradish peroxidase (HRP)–conjugated anti-IgG1, HRP-conjugated anti-IgG2a, HRP-conjugated anti-IgG2b and HRP-conjugated anti-IgG3 by enzyme-linked immunosorbent assay (ELISA), to determine its IgG subclass. (B) IgG from clone A6-9 (2 µg/mL) was serially diluted by 2-fold, and then reacted with GP1e in parallel with control IgG (2 µg/mL) to determine its lowest concentration for GP binding using ELISA. OD, optical density. Fig. 5. Evaluation of glycoprotein (GP)-specific IgG subclass (A) and its lowest concentration for GP binding (B). (A) Purified IgG (2 µg/mL) from clone A6-9 was reacted with GP1e, followed by incubation with horseradish peroxidase (HRP)–conjugated anti- IgG1, HRP-conjugated anti-IgG2a, HRP-conjugated anti-IgG2b and HRP-conjugated anti-IgG3 by enzyme-linked… Clin Exp Vaccine Res. 2017 Jul;6(2):135-145. https://doi.org/10.7774/cevr.2017.6.2.135