VITAMIN D MET Extraction Plate Pos. pressure processor

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Presentation transcript:

VITAMIN D MET Extraction Plate Pos. pressure processor Clinical Diagnostics: 25-OH Vitamin D2 & D3 in Human Plasma Application Note: VDKT10001 VITAMIN D METABOLITE EXTRACTION KIT Compounds : 1,25-DOH Vitamin D3 25-OH Vitamin D2 25-OH Vitamin D3 VITAMIN D KIT EXTRACTION METHOD: (Vitamin D Metabolite Extraction kit: VDKT2C-30) Automated Process using Oroflex Personal Pipettor Reagent Reservoir Internal Std Plate VITAMIN D MET Extraction Plate Serum Sample Plate Station 4 Station 3 Station 2 Station 1 Step1: transfer reagent to extraction plate Step 2: Spike samples with Int. std. and mix sample 3x. Step 3: Transfer sample the Plate Pos. pressure processor LC-MS/MS ANALYSIS (total process time is less than 20 min) RESULTS: 1,25-dihydroxy VD3 25-OH VD2 25-OH VD3 Recovery% 68 69 77 CV% 14 8 6 Orochem Technologies Inc. www.orochem.com www.orochem.com

Phospholipid Profile for Serum Samples using Different Sample Preparation Products