From: Blue Light–Induced Generation of Reactive Oxygen Species in Photoreceptor Ellipsoids Requires Mitochondrial Electron Transport Invest. Ophthalmol. Vis. Sci.. 2003;44(3):1312-1319. doi:10.1167/iovs.02-0768 Figure Legend: Light induced the formation of oxidative products of DAB. (A1, A2) Cells exposed to blue light (480 ± 10 nm; 10 mW/cm2) for 30 minutes displayed a significant amount of precipitate, the oxidative product of DAB (A2), compared with the control (A1). (A3) The formation of the DAB oxidation product was light dependent. Blue light was directed onto one well of a 96-well plate through an objective lens. The cells that were directly exposed to light showed a significant amount of precipitate; by contrast, little precipitate was seen in the region that was outside the projection field of the lens. A sharp contrast was seen at the boundary of the irradiated area (dotted line). (A4) The production of DAB oxidation product was significantly reduced when the cells were pretreated with 1% buffered formalin for 1 hour before exposure to light+DAB, suggesting that the light-induced formation of oxidative polymer may be enzyme mediated. (B) Light-induced precipitate was seen in the ellipsoids of the photoreceptors (arrowheads) and in the cell debris. Magnification, ×400; bar, 10 μm. Date of download: 10/22/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.