Introduction to Microscopy

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Presentation transcript:

Introduction to Microscopy VIBS 443/602

Use your atlas! Use your atlas! Use your atlas! pancreas blood stomach Atlas of Normal Histology by DiFiore testis

Getting Started in the Laboratory Light microscope parts page 24 Kohler illumination pages 34-35

Kohler illumination (order is important) Focus specimen Close field diaphragm Focus condenser Center condenser Open field diaphragm

Kohler illumination Focus specimen Close field diaphragm Focus condenser Center condenser Open field diaphragm

MAGNIFICATION VS. RESOLUTION MAGNIFICATION - INCREASE IN IMAGE SIZE 2. RESOLUTION - SMALLEST DISTANCE BETWEEN TWO POINTS THAT CAN BE SEEN (DISTINGUISHED) CALCULATED BY 0.61 (WAVELENGTH)/NUMERICAL APERTURE 0.25 um FOR LIGHT MICROSCOPE 0.1 nm FOR ELECTRON MICROSCOPE

Peripheral blood smear (May-Grunwald-Giemsa) 113 Peripheral blood smear (May-Grunwald-Giemsa)

Peripheral blood smear (Leishman-Giemsa) 110 Peripheral blood smear (Leishman-Giemsa)

Peripheral blood smear (Leishman-Giemsa) 110 Peripheral blood smear (Leishman-Giemsa)

Peripheral blood smear (Leishman-Giemsa) 110 Peripheral blood smear (Leishman-Giemsa)

Peripheral blood smear (Leishman-Giemsa) eosinophil, basophil, and neutrophils 110

Peripheral blood smear (Leishman-Giemsa) eosinophil, basophil, and neutrophils 110

Pancreas 158

Pancreas 158

Pancreas, monkey (toluidine blue) 156 Pancreas, monkey (toluidine blue)

Pancreas, monkey (toluidine blue) 156 Pancreas, monkey (toluidine blue)

165 UT165 larger secondary spermatocytes and smaller Golgi phase spermatids human testis

Testis - blood and lymph vessels 19680 Testis - blood and lymph vessels

19709 Transparency of unstained tissue

19709 Transparency of unstained tissue

Mast cell in unstained tissue 19709 Mast cell in unstained tissue

Mast cells in stained testicular tissue 19709

Mast cells in stained testicular tissue 19709 Mast cells in stained testicular tissue

145 Fundic stomach

145 Fundic stomach

Argentaffin cells of Fundic stomach, rabbit (toluidine blue) 244 Argentaffin cells of Fundic stomach, rabbit (toluidine blue)

Mucus neck cells Fundic stomach, rabbit (toluidine blue) 244 Mucus neck cells Fundic stomach, rabbit (toluidine blue)

EM 8f EM 12a EM 4c EM 6a Compare sizes of membranes ribosomes mitochondria EM 2b EM 7

EM 8f: Peripheral blood cells; 9,000x Monocyte Lymphocyte Neutrophil

EM 12a: Bone marrow; 13,200x. Note the reticular cell and developing red blood cells.

EM 4c: Intestinal absorption cell; 60,000x Budding RER Coated vesicle Golgi Mitochondria Nucleus Plasma membrane Primary lysosome

EM 2b: Liver; 60,000x; cytoskeletal elements. Microtubes, microfilaments, and intermediate filaments can be compared in this cell, which has a high concentration of cortical microfilaments. Microtube Microfilaments Intermediate filaments

EM 7: Ascites fluid; 80,000x. Clear examples of Golgi apparatuses with their cisternae and vesicles are present in this cell Golgi apparatus Ribosomes Lipofuscin Mitochondrion

EM 6a: Centriole-microtubules; 200,000x EM 6a: Centriole-microtubules; 200,000x. Centriolar region of a cell showing both the stable, triplet microtubule arrays within the centriole, and the labile, individual microtubules originating from pericentriolar material. Centriole Stable microtubule Labile microtubule

EM 4c 60,000x EM 8f 9,000x Compare sizes of membranes ribosomes mitochondria EM 12a 13,200x

EM 6a 200,000x EM 2b 60,000x Compare sizes of membranes ribosomes mitochondria EM 7 80,000x

Conventional TEM, SEM carbon replica TEM EM 2b EM 4a EM 18b EM 6 EM 7 EM 8

Conventional TEM, SEM carbon replica TEM EM 2b EM 4a EM 18b EM 6 EM 7 EM 8

Conventional TEM, SEM carbon replica TEM EM 2b EM 4a EM 18b EM 6 EM 7 EM 8

Conventional TEM, SEM carbon replica TEM EM 2b EM 4a EM 18b EM 6 EM 7 EM 8

Use your atlas! Use your atlas! pancreas blood stomach Atlas of Normal Histology by DiFiore testis

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